RIKEN, Center for Sustainable Resource Science, Yokohama, Kanagawa, Japan.
Zhejiang A & F University, School of Forestry and Biotechnology, Hangzhou, China.
Methods Mol Biol. 2021;2213:17-27. doi: 10.1007/978-1-0716-0954-5_2.
After germination, plants determine their morphogenesis, such as hypocotyl elongation and cotyledon opening, by responding to various wavelengths of light (photomorphogenesis). Cryptochrome is a blue-light photoreceptor that controls de-etiolation, stomatal opening and closing, flowering time, and shade avoidance. Successful incorporation of these phenotypes as indicators into a chemical screening system results in faster selection of candidate compounds. Here, we describe phenotypic screening for the blue-light response of Arabidopsis thaliana seedling and the resulting process that clarifies that the compound obtained in the screening is an inhibitor of cryptochromes.
种子发芽后，植物通过对不同波长光（光形态建成）的反应来决定其形态发生，例如下胚轴伸长和子叶张开。隐花色素是一种蓝光光受体，它控制去黄化、气孔开闭、开花时间和避荫。成功地将这些表型作为指标纳入化学筛选系统中，可以更快地选择候选化合物。在这里，我们描述了拟南芥幼苗对蓝光反应的表型筛选，以及由此阐明在筛选中获得的化合物是隐花色素抑制剂的过程。
