采用 Tc-Cys-Annexin V 凋亡显像技术检测兔激素性股骨头坏死。

Early detection of steroid-induced femoral head necrosis using Tc-Cys-Annexin V-based apoptosis imaging in a rabbit model.

机构信息

Department of Hand and Foot Microsurgery, Second Affiliated Hospital of Inner Mongolia Medical University, No. 1 Yingfang Road, Hohhot, 010030, China.

Department of Nuclear Medicine, Inner Mongolia Medical University Affiliated Hospital, No. 1 Tongdao North Street, Hohhot, 010050, China.

出版信息

Mol Med. 2020 Dec 3;26(1):120. doi: 10.1186/s10020-020-00248-1.

DOI:10.1186/s10020-020-00248-1

PMID:33272196

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7711260/

Abstract

BACKGROUND

At present, the early diagnosis of femoral head necrosis mainly relies on Magnetic resonance imaging (MRI), and most early patients are difficult to make an accurate diagnosis. Therefore, to investigate the early diagnostic value of Tc-Cys-Annexin V Single-photon emission computed tomography (SPECT) imaging were compared with MRI in rabbit models of steroid-induced femoral head necrosis.

METHODS

The animal model of steroid-induced femoral head necrosis (SIFHN) was established in 5-month-old healthy New Zealand white rabbits by injecting horse serum into ear vein and methylprednisolone into gluteal muscle, the purpose of modeling is to simulate the actual clinical situation of SIFNH. Tc-Cys-Annexin V SPECT imaging and MRI were performed at 2nd week, 4th week, and 6th week after modeling. After that, histopathology was used to verify the success of modeling. Apoptosis was detected by transmission electron microscopy (TEM) and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay (TUNEL).

RESULTS

At 2 weeks after the injection of hormone, Tc-Cys-Annexin V SPECT image showed abnormal radioactive uptake in the bilateral femoral head. And over time, the radioactivity concentration was more obvious, and the ratio of T/NT (target tissue/non-target tissues, which is the ratio of femoral head and the ipsilateral femoral shaft) was gradually increased. In the Tc-Cys-Annexin V SPECT imaging at each time point, T/NT ratio of the model group was significantly higher than that of the control group (P < 0.01); at 4 weeks after the injection of hormone, MRI showed an abnormal signal of osteonecrosis. At 2, 4, and 6 weeks after hormone injection, apoptosis was observed by TUNEL and TEM.

CONCLUSIONS

Tc-Cys-Annexin V SPECT imaging can diagnose steroid-induced femoral head necrosis earlier than MRI, and has potential application value for non-invasively detecting early and even ultra-early stage of femoral head necrosis.

摘要

背景

目前，股骨头坏死的早期诊断主要依赖于磁共振成像（MRI），大多数早期患者难以做出准确的诊断。因此，探讨 Tc-Cys-膜联蛋白 V 单光子发射计算机断层扫描（SPECT）成像在兔激素诱导性股骨头坏死模型中的早期诊断价值，并与 MRI 进行比较。

方法

通过静脉注射马血清和臀部肌肉注射甲基强的松龙，建立 5 月龄健康新西兰大白兔激素诱导性股骨头坏死（SIFHN）动物模型，其目的是模拟 SIFNH 的实际临床情况。在建模后第 2 周、第 4 周和第 6 周分别进行 Tc-Cys-膜联蛋白 V SPECT 成像和 MRI 检查，之后通过组织病理学验证建模的成功。通过透射电镜（TEM）和末端脱氧核苷酸转移酶介导的 dUTP-生物素切口末端标记（TUNEL）检测凋亡。

结果

激素注射后 2 周，Tc-Cys-膜联蛋白 V SPECT 图像显示双侧股骨头放射性摄取异常。随着时间的推移，放射性浓度更加明显，目标组织/非目标组织（T/NT）比值逐渐增加。在 Tc-Cys-膜联蛋白 V SPECT 成像的各个时间点，模型组的 T/NT 比值明显高于对照组（P<0.01）；激素注射后 4 周，MRI 显示骨坏死异常信号。在激素注射后 2、4 和 6 周，通过 TUNEL 和 TEM 观察到凋亡。