Department of Biochemistry, Bahauddin Zakariya University, Multan, 60800, Pakistan.
HEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.
Sci Rep. 2020 Dec 3;10(1):21077. doi: 10.1038/s41598-020-78115-6.
The tellurium doped zinc imidazole framework (Te@ZIF-8) is prepared by a two-step hydrothermal strategy for the electrochemical sensing of hydrogen peroxide. Material is characterized by transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The electrochemical characterization of the MOF modified electrode is done by a three-electrode system. Electrochemical sensing of hydrogen peroxide is made by cyclic voltammetry, amperometry, and impedance measurements. Results demonstrate that Te@ZIF-8 shows a detection limit of 60 µM with linearity up to 0.98855. Material is stable to 1000 cycles with no significant change in electrochemical response. Amperometry depicts the recovery of hydrogen peroxide from human serum up to 101%. Impedance curve reveals the surface of Te@ZIF-8-GCE (glassy carbon electrode) as porous and rough and an interface is developed between analyte ions and the sensing material. Finally, the modified electrode is used for the quantitative determination of hydrogen peroxide from serum samples of pancreatic cancer patients, diagnosed with CA 19-9.
碲掺杂锌咪唑骨架（Te@ZIF-8）通过两步水热策略制备，用于过氧化氢的电化学传感。材料通过透射电子显微镜（TEM）、能谱（EDS）、X 射线衍射（XRD）、热重分析（TGA）和差示扫描量热法（DSC）进行了表征。通过三电极系统对 MOF 修饰电极进行了电化学表征。通过循环伏安法、安培法和阻抗测量进行了过氧化氢的电化学传感。结果表明，Te@ZIF-8 的检测限为 60µM，线性度高达 0.98855。材料在 1000 次循环后仍保持稳定，电化学响应无明显变化。安培法显示从人血清中还原过氧化氢的回收率高达 101%。阻抗曲线显示 Te@ZIF-8-GCE（玻碳电极）表面多孔且粗糙，分析物离子与传感材料之间形成了界面。最后，该修饰电极用于定量测定胰腺癌患者血清样品中的过氧化氢，这些患者通过 CA 19-9 进行了诊断。
