Fatima Batool, Hussain Dilshad, Bashir Shazia, Hussain Hafiza Tanzilla, Aslam Rashida, Nawaz Rahat, Rashid Hafiza Nadia, Bashir Nabila, Majeed Saadat, Ashiq Muhammad Naeem, Najam-Ul-Haq Muhammad
Department of Biochemistry, Bahauddin Zakariya University, Multan 60800, Pakistan.
Division of Analytical Chemistry, Institute of Chemical Sciences, BahauddinZakariya University, Multan 60800, Pakistan; HEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi 75270, Pakistan.
Mater Sci Eng C Mater Biol Appl. 2020 Dec;117:111296. doi: 10.1016/j.msec.2020.111296. Epub 2020 Jul 24.
A selective and cost-effective biosensor based on catalase immobilized antimonene quantum dots modified glassy carbon electrode (Cat@AMQDs-GCE) is designed for the first time to determine hydrogen peroxide (HO). Antimonene quantum dots (AMQDs) are synthesized by a single step method, characterized by various analytical techniques and applied to the electrochemical sensing of hydrogen peroxide. Catalase enzyme specific for HO reduction is immobilized onto AMQDs to facilitate its detection by cyclic voltammetry and amperometry. Concentration, scan rate, pH, stability and selectivity are optimized. Linearity of Cat@AMQDs-GCE is determined as 0.989 with limit of detection as 4.4 μM. Amperometric measurements show recovery of 95 to 103.4% for HO from human serum samples. Cat@AMQDs-GCE is electrochemically stable up to 30 cycles, reducing the cost of analysis. Cat@AMQDs-GCE shows good selectivity in presence of ascorbic acid, dopamine, leucine and glucose. Prepared electrode is also applied for the quantitative determination of HO from ovarian cancer serum. CA 125 concentration is previously determined by Elecsys CA 125 II Assay. Results demonstrate that concentration of HO increases with increasing levels of CA125 in serum.
首次设计了一种基于过氧化氢酶固定化锑烯量子点修饰玻碳电极（Cat@AMQDs-GCE）的选择性且经济高效的生物传感器，用于测定过氧化氢（H₂O₂）。通过一步法合成锑烯量子点（AMQDs），采用多种分析技术对其进行表征，并将其应用于过氧化氢的电化学传感。将对H₂O₂还原具有特异性的过氧化氢酶固定在AMQDs上，以便通过循环伏安法和安培法对其进行检测。对浓度、扫描速率、pH值、稳定性和选择性进行了优化。Cat@AMQDs-GCE的线性度确定为0.989，检测限为4.4 μM。安培测量显示，人血清样品中H₂O₂的回收率为95%至103.4%。Cat@AMQDs-GCE在高达30个循环的情况下具有电化学稳定性，降低了分析成本。Cat@AMQDs-GCE在抗坏血酸、多巴胺、亮氨酸和葡萄糖存在下表现出良好的选择性。制备的电极还用于定量测定卵巢癌血清中的H₂O₂。CA 125浓度先前通过Elecsys CA 125 II测定法测定。结果表明，血清中H₂O₂的浓度随CA125水平的升高而增加。
