Department of Biotechnology, Institute of Biology and Biotechnology, University of Rzeszów, Pigonia 1, 35-310, Rzeszów, Poland.
Sci Rep. 2020 Dec 3;10(1):21212. doi: 10.1038/s41598-020-78177-6.
The aim of this study was to investigate the dissipation kinetics of fluxapyroxad in apples, the influence of biological treatment with yeast, and the estimation of dietary exposure for consumers, both adults and children. The gas chromatography technique with the electron capture detector was used to analyse the fluxapyroxad residues. Samples of apples were prepared by the quick, easy, cheap, effective, rugged and safe (QuEChERS) method. The average fluxapyroxad recoveries in apple samples ranged from 107.9 to 118.4%, the relative standard deviations ranged from 4.2 to 4.7%, and the limit of quantification was 0.005 mg/kg. The dissipation half-lives in Gala and Idared varieties were 8.9 and 9.0 days, respectively. Degradation levels of the tested active substance after application of yeast included in a biological preparation Myco-Sin were 59.9% for Gala and 43.8% for Idared. The estimated dietary risk for fluxapyroxad in apples was on the acceptable safety level (below 9.8% for children and 1.9% for adults) and does not pose a danger to the health of consumers.
本研究旨在调查氟吡菌酰胺在苹果中的消解动态、酵母生物处理的影响，以及成人和儿童消费者的膳食暴露估计。采用带电子捕获检测器的气相色谱技术分析氟吡菌酰胺残留。采用快速、简便、廉价、有效、耐用和安全（QuEChERS）方法制备苹果样品。苹果样品中氟吡菌酰胺的平均回收率为 107.9%至 118.4%，相对标准偏差为 4.2%至 4.7%，定量限为 0.005 mg/kg。在 Gala 和 Idared 品种中的消解半衰期分别为 8.9 和 9.0 天。应用包括在生物制剂 Myco-Sin 中的酵母后，测试的活性物质的降解水平分别为 Gala 的 59.9%和 Idared 的 43.8%。苹果中氟吡菌酰胺的估计膳食风险处于可接受的安全水平（儿童低于 9.8%，成人低于 1.9%），不会对消费者健康构成威胁。
