酵母YC2对二甲戊灵的降解及其两种主要代谢产物的测定
Degradation of pendimethalin by the yeast YC2 and determination of its two main metabolites.
作者信息
Han Yajie, Tang Zonggui, Bao Huifang, Wu Dongmei, Deng Xiaolin, Guo Gaowei, Ye Bang-Ce, Dai Bin
机构信息
School of Chemistry and Chemical Engineering/The Key Lab. for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University Shihezi 832003 People's Republic of China
College of Life Science, Shihezi University Shihezi 832003 People's Republic of China.
出版信息
RSC Adv. 2019 Jan 2;9(1):491-497. doi: 10.1039/c8ra07872f. eCollection 2018 Dec 19.
In this study, we isolated a yeast strain, YC2, by enrichment culture from pendimethalin-contaminated soil. The analysis of its phenotypic features and 26S rRNA sequence confirmed that the strain YC2 is . According to the kinetics of pendimethalin degradation, YC2 could degrade 74% of 200 mg L pendimethalin in CMB liquid culture over 8 days. LC-MS/MS was used to identify the metabolites of pendimethalin after degradation. This study confirmed that its metabolites consist of 1,2-dimethyl-3,5-dinitro-4-(buta-1,3-dien-2-yl)-dinitrobenzenamine--oxide and 1,2-dimethyl-3,5-dinitro-4-(prop-1-en-2-yl)-dinitrobenzenamine--oxide, which were broken down by a series of enzymatic reactions to produce CO and HO. Thus, the study herein sheds light on the role of yeast in the degradation of pendimethalin.
在本研究中，我们通过富集培养从二甲戊灵污染的土壤中分离出一株酵母菌株YC2。对其表型特征和26S rRNA序列的分析证实，菌株YC2为 。根据二甲戊灵的降解动力学，YC2在CMB液体培养中8天内可降解200 mg/L二甲戊灵的74%。采用LC-MS/MS鉴定二甲戊灵降解后的代谢产物。本研究证实其代谢产物由1,2-二甲基-3,5-二硝基-4-(丁-1,3-二烯-2-基)-二硝基苯甲胺-氧化物和1,2-二甲基-3,5-二硝基-4-(丙-1-烯-2-基)-二硝基苯甲胺-氧化物组成，它们通过一系列酶促反应分解产生CO₂和H₂O。因此，本研究揭示了酵母在二甲戊灵降解中的作用。
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