Pak J Biol Sci. 2020 Jan;23(12):1539-1550. doi: 10.3923/pjbs.2020.1539.1550.
Molecular genetic methods show conclusive turn in phylogeny investigations and therefore, understanding the allocation and extension of genetic diversity inside and among species. The current study aims to evaluate genetic diversity using molecular markers (5 SSR and 5 ISSR) on 13 sunflowers (Helianthus annuus) genotypes from different regions.
The data acquired with the highest degree of polymorphism were confirmed. Polymorphic bands (24 and 55) were demonstrated totally through SSR and ISSR, respectively. Nevertheless, ISSR was superior in exhibiting polymorphism rate (97.37%) compared to SSR markers (90.83%). A range from 2-21 of amplified fragments, with molecular weights varying from 34-1331 bp, was noticeable. The genetic similarity coefficient was employed to set UPGMA dendrogram.
Dissimilarity was found in genotypes clustering within groups, whilst the manner of clustering in genotypes kept adjacent in ISSR and aggregated results of SSR and ISSR. Dice similarity ranged from 0.058-0.792, 0.168-0.770 and 0.154-0.847 with SSR, ISSR and the combined phylogeny dendrogram, respectively. The genetic variance of sunflower accessions of wild and cultivated cultivars from different geographic regions was established.
Knowledge of these results could be applied to confirm a wide genetic basis for outlook sunflower and manage germplasm breeding programs.
分子遗传方法在系统发育研究中显示出明确的转折，因此，理解遗传多样性在物种内和物种间的分配和扩展。本研究旨在使用分子标记（5 个 SSR 和 5 个 ISSR）评估来自不同地区的 13 个向日葵（Helianthus annuus）基因型的遗传多样性。
确认获得了最高程度多态性的数据。SSR 和 ISSR 分别显示了完全多态性带（24 和 55）。然而，与 SSR 标记（90.83%）相比，ISSR 表现出更高的多态性率（97.37%）。可以观察到扩增片段的分子量从 34-1331bp 不等，范围从 2-21 个。遗传相似系数用于构建 UPGMA 树状图。
在组内基因型聚类中发现了相似性，而在 ISSR 中基因型聚类的方式与 SSR 和 ISSR 聚合结果相邻。Dice 相似性分别为 0.058-0.792、0.168-0.770 和 0.154-0.847，与 SSR、ISSR 和综合系统发育树状图相对应。建立了来自不同地理区域的野生和栽培品种向日葵材料的遗传方差。
了解这些结果可以应用于确认外观向日葵的广泛遗传基础，并管理种质资源培育计划。
