Pak J Biol Sci. 2020 Jan;23(12):1591-1600. doi: 10.3923/pjbs.2020.1591.1600.
In Egypt, Nile tilapia represents the main cultured type due to its economical price, palatability and easy culturing. This study was aimed to elucidate the pathogenicity of V. alginolyticus isolated from diseased sea bass and experimentally infected healthy Nile tilapia fish.
Healthy Nile tilapia fish were injected I/P with V. alginolyticus isolated from diseased sea bass. Symptoms and mortality rates of infected Nile tilapia fish were recorded during the experimental period. Re-isolation of V. alginolyticus was done from infected tilapia fish by bacteriological methods. For confirmation the pathogenicity of Vibrio isolated either from marine fish or tilapia fish, PCR test was done using tdh and bla gens. Liver and kidney function tests with histopathological examinations of some organs were performed. Treatment trial was done according to the antibiotic sensitivity test.
The isolated Vibrio is highly pathogenic to Nile tilapia fish causing deterioration in all parameters which finished by severe mortalities. Treatment with florfenicol, enrofloxacin, or oxytetracycline reduced the mortality rate and improved liver and kidney function parameters of infected Nile tilapia fish.
V. alginolyticus can infect both marine and fresh water fish inducing a high mortality rate. Treatment of infected fish with florfenicol, enrofloxacin, or oxytetracycline reduces the mortality rate.
在埃及，尼罗罗非鱼由于其经济价格、可食用性和易于养殖，成为主要养殖品种。本研究旨在阐明从患病海鲈中分离出的溶藻弧菌对健康尼罗罗非鱼的致病性，并进行了实验感染。
将从患病海鲈中分离出的溶藻弧菌通过 I/P 注射到健康的尼罗罗非鱼体内。在实验期间，记录感染尼罗罗非鱼的症状和死亡率。通过细菌学方法从感染的罗非鱼中重新分离溶藻弧菌。为了确认从海洋鱼类或罗非鱼中分离出的弧菌的致病性，使用 tdh 和 bla 基因进行了 PCR 测试。对部分器官进行了肝肾功能检查和组织病理学检查。根据抗生素敏感性试验进行了治疗试验。
分离出的弧菌对尼罗罗非鱼具有高度致病性，导致所有参数恶化，最终死亡率很高。用氟苯尼考、恩诺沙星或土霉素治疗可降低感染尼罗罗非鱼的死亡率，并改善肝肾功能参数。
溶藻弧菌可感染海水鱼和淡水鱼，引起高死亡率。用氟苯尼考、恩诺沙星或土霉素治疗感染鱼可降低死亡率。
