Laboratoire d'Analyse, Traitement et Valorisation des Polluants de l'Environnement et des Produits, Département de Microbiologie, Faculté de Pharmacie, Rue Avicenne, 5000, Monastir, Tunisia.
Folia Microbiol (Praha). 2013 Jan;58(1):17-26. doi: 10.1007/s12223-012-0174-x. Epub 2012 Jun 9.
The genus Vibrio is characterized by a large number of species and some of them are human pathogens causing gastrointestinal and wound infections through the ingestion or manipulation of contaminated fishes and shellfish including Vibrio parahaemolyticus and Vibrio alginolyticus. In this study, we reported the phenotypic and molecular characterization of 9 V. parahaemolyticus and 27 V. alginolyticus strains isolated from outbreaks affecting cultured Gilthead sea bream (Sparus aurata L.) and Sea bass (Dicentrarchus labrax) along the Tunisian coast from 2008 to 2009. All isolates were tested for the presence of DNase, caseinase, protease, lipase, amylase, gelatinase, hemolytic activity and antibacterial resistance to different drugs. Randomly amplified polymorphic DNA was used to examine the genetic relatedness among the V. parahaemolyticus and V. alginolyticus strains.
弧菌属的特点是物种数量众多，其中一些是人类病原体，通过摄入或处理受污染的鱼类和贝类（包括副溶血性弧菌和 alginolyticus 弧菌）引起胃肠道和伤口感染。在本研究中，我们报告了 9 株副溶血性弧菌和 27 株 alginolyticus 弧菌菌株的表型和分子特征，这些菌株是从 2008 年至 2009 年期间突尼斯沿海受影响的养殖金头鲷（Sparus aurata L.）和海鲈（Dicentrarchus labrax）爆发中分离出来的。所有分离株均进行了 DNase、caseinase、蛋白酶、脂肪酶、淀粉酶、明胶酶、溶血活性和对不同药物的抗菌耐药性检测。随机扩增多态性 DNA 用于检测副溶血性弧菌和 alginolyticus 弧菌菌株之间的遗传相关性。
