鹅胸腺发育和退化相关的 lncRNAs、miRNAs 和 mRNAs 的综合分析。
Comprehensive analysis of lncRNAs, miRNAs and mRNAs related to thymic development and involution in goose.
机构信息
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China.
Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China.
出版信息
Genomics. 2021 Jan;113(1 Pt 2):1176-1188. doi: 10.1016/j.ygeno.2020.11.031. Epub 2020 Dec 2.
Thymic involution is a sign of immunosenescence, but little is known about it in goose. miRNAs and lncRNAs are critical factors regulating organ growth and development. In this study, we comprehensively analyzed the profiles of lncRNAs, miRNAs and mRNAs during the development and involution of the thymus in Magang goose. The results showed that 2436 genes, 16 miRNAs and 417 lncRNAs were differentially co-expressed between the developmental (20-embryo age, 3-day post-hatch and 3-month age) and degenerative (6-month age) stages. The functional analysis showed that these differentially expressed genes were significantly enriched in cell proliferation, cell adhesion, apoptotic signaling pathway, and Notch signaling pathway. In addition, we established a gene-gene network through the STRING database and identified 50 key genes. Finally, we constructed a miRNA-mRNA network followed by a lncRNA-miRNA-mRNA network. These results suggest that lncRNAs and miRNAs may be involved in the regulation of thymic development and involution in goose.
胸腺萎缩是免疫衰老的标志，但关于鹅的胸腺萎缩知之甚少。miRNAs 和 lncRNAs 是调控器官生长发育的关键因素。在这项研究中，我们全面分析了 Magang 鹅胸腺发育和萎缩过程中 lncRNAs、miRNAs 和 mRNAs 的表达谱。结果表明，在发育阶段（20 胚龄、3 日龄和 3 月龄）和退行性阶段（6 月龄）之间，有 2436 个基因、16 个 miRNAs 和 417 个 lncRNAs 存在差异共表达。功能分析表明，这些差异表达基因在细胞增殖、细胞黏附、凋亡信号通路和 Notch 信号通路中显著富集。此外，我们通过 STRING 数据库构建了一个基因-基因网络，鉴定出 50 个关键基因。最后，我们构建了一个 miRNA-mRNA 网络，随后构建了一个 lncRNA-miRNA-mRNA 网络。这些结果表明，lncRNAs 和 miRNAs 可能参与了鹅胸腺发育和萎缩的调控。
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