characterizing the expression profiles of circular RNA in the age-related involution of the thymus in the Magang goose
Characterization of circular RNA expression profiles in the age-related thymic involution of Magang goose.
机构信息
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China.
College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China; Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China.
出版信息
Dev Comp Immunol. 2023 Feb;139:104581. doi: 10.1016/j.dci.2022.104581. Epub 2022 Oct 22.
The thymus is a vital immune organ, but its function gradually declines with age. Circular RNAs (circRNAs) are related to the development of tissues and organs. In this study, bioinformatics analysis showed that 1329, 755, and 417 circRNAs were differentially expressed between the comparison groups of 6-month age (M6) and 20-embryo age (E20), 3-day post-hatch (P3), and 3-month age (M3) Magang geese, respectively. Among them, 167 circRNAs were differentially co-expressed between thymic development (E20, P3, and M3) and involution (M6). Functional analysis showed significant enrichment of phosphorylation and positive regulation of GTPase activity. Furthermore, pathway analysis has shown that glycerolipid metabolism and the Wnt signaling pathway are critical pathways in the thymic involution process. Finally, we constructed the competitive endogenous RNA (ceRNA) network. The results of this study suggest that circRNAs may be involved in the age-related thymic involution of the Magang goose.
胸腺是一个重要的免疫器官，但它的功能随着年龄的增长而逐渐下降。环状 RNA（circRNA）与组织和器官的发育有关。在这项研究中，生物信息学分析表明，在 Magang 鹅的月龄为 6 个月（M6）和 20 胚龄（E20）、出雏后 3 天（P3）和 3 个月（M3）的对照组之间，有 1329、755 和 417 个 circRNA 存在差异表达。其中，167 个 circRNA 在胸腺发育（E20、P3 和 M3）和退化（M6）之间存在差异共表达。功能分析表明，磷酸化和 GTPase 活性的正调控显著富集。此外，通路分析表明，甘油酯代谢和 Wnt 信号通路是胸腺退化过程中的关键通路。最后，我们构建了竞争性内源 RNA（ceRNA）网络。这项研究的结果表明，circRNA 可能参与了 Magang 鹅的年龄相关胸腺退化。
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