Voloshin S A, Feyzkhanova G U, Savvateeva E N, Smoldovskaya O V, Rubina A Yu
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991 Russia.
Mol Biol (Mosk). 2020 Nov-Dec;54(6):1046-1056. doi: 10.31857/S0026898420060130.
Cytokines and acute phase proteins play an important role in the development of the immune response during inflammatory reactions. Depending on the type of disease, the development of inflammation is accompanied by changes in concentrations (both decrease and increase) of not one, but many inflammatory biomarkers. Here, a quantitative microarray-based method for multiplex immunoassay of eight biomarkers of human inflammation, namely acute phase proteins (C-reactive protein, serum amyloid protein A) and cytokines (IL-6, IL-8, IL-17, IL-18, IP10/CXCL10, TNFα) was developed and the possibility of its use for the detection of inflammatory biomarkers in a culture medium has been demonstrated. The developed method can be used to evaluate changes of the inflammatory biomarker profile induced by different agents or to determine the concentrations of biomarkers after activation of cells while studying different diseases with the help of in vitro models.
细胞因子和急性期蛋白在炎症反应期间免疫应答的发展中起重要作用。根据疾病类型，炎症的发展伴随着多种而非单一炎症生物标志物浓度的变化（包括降低和升高）。在此，开发了一种基于定量微阵列的方法，用于对人炎症的八种生物标志物进行多重免疫测定，即急性期蛋白（C反应蛋白、血清淀粉样蛋白A）和细胞因子（IL-6、IL-8、IL-17、IL-18、IP10/CXCL10、TNFα），并证明了其用于检测培养基中炎症生物标志物的可能性。所开发的方法可用于评估不同试剂诱导的炎症生物标志物谱的变化，或在借助体外模型研究不同疾病时确定细胞激活后生物标志物的浓度。
