Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
Department of Clinical Sciences, Faculty of Veterinary Medicine, Semnan University, Semnan, Iran.
J Equine Vet Sci. 2020 Dec;95:103273. doi: 10.1016/j.jevs.2020.103273. Epub 2020 Sep 30.
This investigation aimed to compare the cell-free fetal DNA (cffDNA) plasma present in three trimesters of pregnancy in Torkaman pregnant mare. Peripheral blood samples of 32 pregnant mares in three trimesters of pregnancy were collected in tubes containing ethylenediaminetetraacetic acid at three time points. Circulating cffDNA was extracted from 3 mL of maternal plasma. Using outer and inner primers, a conventional polymerase chain reaction was performed for the sex-determining region Y (SRY) gene present in the Y chromosome. Of the total 32 Torkaman pregnant mares, 24 were carrying male fetuses and eight were carrying female fetuses. In total, the accuracy of the test was 48.75%, 68.75%, and 75% in the first, second, and third trimesters of pregnancy, respectively. The sensitivities were 25%, 58.32%, and 66.66%, respectively, whereas their specificities were 100% in all trimesters. In conclusion, the SRY gene can permit the detection of equine fetal sex with good accuracy through cffDNA analysis in maternal plasma just in the third trimester of pregnancy, although specificity in all duration of pregnancy was 100%.
本研究旨在比较托卡曼妊娠母马妊娠三个时期的血浆中游离胎儿 DNA（cffDNA）。在三个时间点，将含有乙二胺四乙酸的管收集 32 头妊娠母马妊娠三个时期的外周血样本。从 3ml 母体血浆中提取循环 cffDNA。使用外引物和内引物，对存在于 Y 染色体上的性别决定区 Y（SRY）基因进行常规聚合酶链反应。在 32 头托卡曼妊娠母马中，24 头怀有雄性胎儿，8 头怀有雌性胎儿。总的来说，该检测在妊娠第一、第二和第三期的准确率分别为 48.75%、68.75%和 75%。灵敏度分别为 25%、58.32%和 66.66%，而在所有孕期的特异性均为 100%。总之，通过母血浆中 cffDNA 分析，SRY 基因可在妊娠晚期（仅在妊娠第三期）准确检测马胎儿性别，尽管在整个孕期特异性均为 100%。
