Mirsafaie Maryam, Kheirollahi Majid, Moghaddam-Banaem Lida
Department of Midwifery and Reproductive Health, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2023 Mar 21;12:69. doi: 10.4103/abr.abr_54_21. eCollection 2023.
This study aimed to investigate the relationship between maternal predisposing factors with the level of maternal serum pregnancy-associated plasma protein A and free subunit human chorionic gonadotropin and nuchal translucency.
We performed a cross-sectional-analytical study on 762 pregnant women who referred to the Gene Azma Medical Genetics Laboratory in Isfahan for amniocentesis. All pregnant women at high risk of screening in the first trimester of pregnancy for trisomy 21 and other aneuploidy were referred to a gynecologist for amniotic fluid sampling (amniocentesis). Multiple of the means (MoM) of PAPPA ≤0.5, 0.5 ≥ MoM free β-hCG >2.5, and NT ≥3.5 mm were considered abnormal. We used Chi-square method and Mann-Whitney U-test to compare data qualitative and quantitative, respectively.
In individuals with less pregnancies and deliveries, the value of abnormal NT was higher ( < 0.01, < 0.001, respectively). On the other hand, the highest abnormal rate of NT was observed in pregnant women under 35 years (21, 84%, < 0.012). In addition, abnormal levels of free -hCG are more common in women < 35 years of age (186, 66.9%, < 0.02) and female fetuses (171, 58.8%) ( < 0.006).
According to the results of this study, it can be said that considering the underlying factors of pregnant mothers in performing tests related to screening in the first trimester of pregnancy can lead to a reduction in false positive rates.
本研究旨在探讨孕妇的易感因素与孕妇血清妊娠相关血浆蛋白A、游离β亚基人绒毛膜促性腺激素水平及颈项透明层之间的关系。
我们对762名前往伊斯法罕基因阿斯玛医学遗传学实验室进行羊膜穿刺术的孕妇进行了横断面分析研究。所有在孕早期有21三体综合征和其他非整倍体筛查高风险的孕妇均被转介给妇科医生进行羊水采样（羊膜穿刺术）。PAPPA的中位数倍数（MoM）≤0.5、0.5≥MoM游离β-hCG>2.5以及NT≥3.5mm被视为异常。我们分别使用卡方检验和曼-惠特尼U检验来比较定性和定量数据。
在妊娠和分娩次数较少的个体中，NT异常值较高（分别为<0.01，<0.001）。另一方面，NT异常率最高的是35岁以下的孕妇（21例，84%，<0.012）。此外，游离β-hCG异常水平在35岁以下的女性（186例，66.9%，<0.02）和女胎（171例，58.8%）中更为常见（<0.006）。
根据本研究结果，可以说在进行孕早期筛查相关检测时考虑孕妇的潜在因素可降低假阳性率。
