Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
National Engineering Research Center for Gelatin-based Traditional Chinese Medicine, Dong-E-E-Jiao Co. Ltd., Liaocheng, Shandong, China.
J Equine Vet Sci. 2020 Dec;95:103279. doi: 10.1016/j.jevs.2020.103279. Epub 2020 Oct 6.
The purpose of this study was to determine the optimal time for ovulation induction and artificial insemination (AI) based on the relationship between estrous behavior and ovulation in jennies. Thirty-two jennies were teased by one jackass for 1 hour per day during 46 days and estrous behaviors were recorded, while the follicular development and ovulation was examined by ultrasound. Furthermore, another 31 jennies were teased by one jackass as the teasing group (group T), which were injected with Deslorelin at 2 and 4 days after the onset of estrus, and AI was performed at 8 hours after each injection. Moreover, Ultrasound was performed on the follicle development of 23 jennies as the ultrasonography group (group U). Injection with Deslorelin when the follicle diameter ≥ 30 mm, and AI was performed at 8 hours later. The results showed that mouth clapping was the specific estrous behavior of jennies and indicated the beginning of estrus. The mean time for jennies to develop dominant follicles (≥30 mm) after the onset of estrus was 3.5 ± 1.3 days, and the mean time between the onset of estrus and ovulation was 5.1 ± 1.5 days. Estrous behaviors ended 0.5 ± 1.2 days after ovulation. After AI, there were no significant differences in ovulation (96.8% vs. 91.3%) and conception rates (40.0% vs. 38.1%) between group T and U. The optimal breeding time of jennies can be determined by jackass teasing and hastening ovulation by Deslorelin injection.
本研究旨在确定根据母马发情行为与排卵的关系，进行排卵诱导和人工授精（AI）的最佳时间。32 匹母马每天被一头公驴挑逗 1 小时，共 46 天，并记录发情行为，同时通过超声检查卵泡发育和排卵情况。此外，另外 31 匹母马作为挑逗组（组 T）被一头公驴挑逗，发情后第 2 天和第 4 天注射地洛瑞林，每次注射后 8 小时进行 AI。此外，对 23 匹母马的卵泡发育进行超声检查作为超声组（组 U）。当卵泡直径≥30mm 时注射地洛瑞林，8 小时后进行 AI。结果表明，口拍手是母马特有的发情行为，表明发情开始。发情后母马发展优势卵泡（≥30mm）的平均时间为 3.5±1.3 天，发情与排卵之间的平均时间为 5.1±1.5 天。发情行为在排卵后 0.5±1.2 天结束。AI 后，组 T 和 U 的排卵率（96.8%比 91.3%）和受孕率（40.0%比 38.1%）无显著差异。通过公驴挑逗和地洛瑞林注射促进排卵，可以确定母马的最佳配种时间。
