• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

呋喃丹加速 spns1 突变型斑马鱼的细胞衰老并缩短其寿命。

Carbofuran accelerates the cellular senescence and declines the life span of spns1 mutant zebrafish.

机构信息

Department of Pharmacy, University of Rajshahi, Rajshahi, Bangladesh.

Department of Molecular Medicine, The Scripps Research Institute, Jupiter, FL, USA.

出版信息

J Cell Mol Med. 2021 Jan;25(2):1048-1059. doi: 10.1111/jcmm.16171. Epub 2020 Dec 4.

DOI:10.1111/jcmm.16171
PMID:33277797
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7812278/
Abstract

Carbofuran is a carbamate pesticide, widely used in agricultural practices to increase crop productivity. In mammals, carbofuran is known to cause several untoward effects, such as apoptosis in the hippocampal neuron, oxidative stress, loss of memory and chromosomal anomalies. Most of these effects are implicated with cellular senescence. Therefore, the present study aimed to determine the effect of carbofuran on cellular senescence and biological ageing. Spinster homolog 1 (Spns1) is a transmembrane transporter, regulates autolysosomal biogenesis and plays a role in cellular senescence and survival. Using senescence-associated β-galactosidase staining, we found that carbofuran accelerates the cellular senescence in spns1 mutant zebrafish. The yolk opaqueness, a premature ageing phenotype in zebrafish embryos, was accelerated by carbofuran treatment. In the survival study, carbofuran shortened the life span of spns1 mutant zebrafish. Autophagy is the cellular lysosomal degradation, usually up-regulated in the senescent cells. To know the impact of carbofuran exposure on autophagy progress, we established a double-transgenic zebrafish line, harbouring EGFP-tagged LC3-II and mCherry-tagged Lamp1 on spns1 mutant background, whereas we found, carbofuran exposure synergistically accelerates autolysosome formation with insufficient lysosome-mediated degradation. Our data collectively suggest that carbofuran exposure synergistically accelerates the cellular senescence and affects biological ageing in spns1 defective animals.

摘要

呋喃丹是一种氨基甲酸酯类农药，广泛应用于农业生产中以提高作物产量。在哺乳动物中，呋喃丹已知会引起多种不良反应，如海马神经元凋亡、氧化应激、记忆丧失和染色体异常。这些影响大多与细胞衰老有关。因此，本研究旨在确定呋喃丹对细胞衰老和生物老化的影响。Spns1 是一种跨膜转运蛋白，调节自噬体的生物发生，在细胞衰老和存活中发挥作用。通过衰老相关的β-半乳糖苷酶染色，我们发现呋喃丹加速了 spns1 突变型斑马鱼的细胞衰老。卵黄不透明度是斑马鱼胚胎的一种早衰表型，呋喃丹处理加速了这种表型。在生存研究中，呋喃丹缩短了 spns1 突变型斑马鱼的寿命。自噬是细胞溶酶体降解，通常在衰老细胞中上调。为了了解呋喃丹暴露对自噬进展的影响，我们建立了一个双转基因斑马鱼系，在 spns1 突变背景下携带 EGFP 标记的 LC3-II 和 mCherry 标记的 Lamp1，而我们发现，呋喃丹暴露协同加速了自噬体的形成，但溶酶体介导的降解不足。我们的数据表明，呋喃丹暴露协同加速了 spns1 缺陷动物的细胞衰老，并影响了它们的生物老化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/0f10eaf5aac0/JCMM-25-1048-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/9d4465b58951/JCMM-25-1048-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/570ce7fb8449/JCMM-25-1048-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/969300f8f5f1/JCMM-25-1048-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/77a257a4520a/JCMM-25-1048-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/ac665d90601f/JCMM-25-1048-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/0f10eaf5aac0/JCMM-25-1048-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/9d4465b58951/JCMM-25-1048-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/570ce7fb8449/JCMM-25-1048-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/969300f8f5f1/JCMM-25-1048-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/77a257a4520a/JCMM-25-1048-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/ac665d90601f/JCMM-25-1048-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b504/7812278/0f10eaf5aac0/JCMM-25-1048-g006.jpg

相似文献

1
Carbofuran accelerates the cellular senescence and declines the life span of spns1 mutant zebrafish.呋喃丹加速 spns1 突变型斑马鱼的细胞衰老并缩短其寿命。
J Cell Mol Med. 2021 Jan;25(2):1048-1059. doi: 10.1111/jcmm.16171. Epub 2020 Dec 4.
2
Carbofuran affects cellular autophagy and developmental senescence through the impairment of Nrf2 signalling.呋喃丹通过损害 Nrf2 信号通路影响细胞自噬和发育性衰老。
J Cell Mol Med. 2022 Jan;26(1):35-47. doi: 10.1111/jcmm.16774. Epub 2021 Jul 9.
3
Autolysosome biogenesis and developmental senescence are regulated by both Spns1 and v-ATPase.自噬溶酶体的生物发生和发育性衰老受Spns1和V-ATP酶共同调控。
Autophagy. 2017 Feb;13(2):386-403. doi: 10.1080/15548627.2016.1256934. Epub 2016 Nov 22.
4
Aberrant autolysosomal regulation is linked to the induction of embryonic senescence: differential roles of Beclin 1 and p53 in vertebrate Spns1 deficiency.异常的自噬溶酶体调节与胚胎衰老的诱导有关：Beclin 1和p53在脊椎动物Spns1缺陷中的不同作用。
PLoS Genet. 2014 Jun 26;10(6):e1004409. doi: 10.1371/journal.pgen.1004409. eCollection 2014 Jun.
5
Deficiency of spns1 exacerbates per- and polyfluoroalkyl substances mediated hepatic toxicity and steatosis in zebrafish (Danio rerio).SPNS1 缺乏会加剧全氟和多氟烷基物质介导的斑马鱼（Danio rerio）肝毒性和脂肪变性。
Toxicology. 2023 Nov;499:153641. doi: 10.1016/j.tox.2023.153641. Epub 2023 Oct 6.
6
Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis.斑马鱼卵黄特异性未真正起始（nrs）基因是果蝇spinster基因的脊椎动物同源物，对胚胎发育至关重要。
Dev Dyn. 2002 Mar;223(2):298-305. doi: 10.1002/dvdy.10060.
7
The identification of zebrafish mutants showing alterations in senescence-associated biomarkers.对衰老相关生物标志物出现改变的斑马鱼突变体的鉴定。
PLoS Genet. 2008 Aug 15;4(8):e1000152. doi: 10.1371/journal.pgen.1000152.
8
Spns1 is a lysophospholipid transporter mediating lysosomal phospholipid salvage.Spns1 是一种溶血磷脂转运蛋白，可介导溶酶体磷脂的再循环。
Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2210353119. doi: 10.1073/pnas.2210353119. Epub 2022 Sep 26.
9
Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress-induced senescence.溶酶体自噬降解胞质染色质片段拮抗氧化应激诱导的衰老。
J Biol Chem. 2020 Apr 3;295(14):4451-4463. doi: 10.1074/jbc.RA119.010734. Epub 2020 Feb 11.
10
Bisphenol A exposure under metabolic stress induces accelerated cellular senescence in vivo in a p53 independent manner.代谢应激下的双酚 A 暴露以 p53 非依赖的方式在体内诱导细胞加速衰老。
Sci Total Environ. 2019 Nov 1;689:1201-1211. doi: 10.1016/j.scitotenv.2019.06.391. Epub 2019 Jun 25.

引用本文的文献

1
Carbofuran pesticide toxicity to the eye.呋喃丹农药对眼睛的毒性。
Exp Eye Res. 2023 Feb;227:109355. doi: 10.1016/j.exer.2022.109355. Epub 2022 Dec 23.
2
Lysosomal Function Impacts the Skeletal Muscle Extracellular Matrix.溶酶体功能影响骨骼肌细胞外基质。
J Dev Biol. 2021 Nov 23;9(4):52. doi: 10.3390/jdb9040052.
3
Carbofuran affects cellular autophagy and developmental senescence through the impairment of Nrf2 signalling.呋喃丹通过损害 Nrf2 信号通路影响细胞自噬和发育性衰老。

本文引用的文献

1
Implications of Oxidative Stress and Cellular Senescence in Age-Related Thymus Involution.氧化应激和细胞衰老对与年龄相关的胸腺萎缩的影响。
Oxid Med Cell Longev. 2020 Feb 5;2020:7986071. doi: 10.1155/2020/7986071. eCollection 2020.
2
Cellular senescence in development, regeneration and disease.细胞衰老与发育、再生和疾病。
Development. 2019 Oct 1;146(20):dev151837. doi: 10.1242/dev.151837.
3
PAI-1 contributes to homocysteine-induced cellular senescence.PAI-1 促进同型半胱氨酸诱导的细胞衰老。
J Cell Mol Med. 2022 Jan;26(1):35-47. doi: 10.1111/jcmm.16774. Epub 2021 Jul 9.
Cell Signal. 2019 Dec;64:109394. doi: 10.1016/j.cellsig.2019.109394. Epub 2019 Aug 28.
4
Studies on the ameliorative effect of curcumin on carbofuran induced perturbations in the activity of lactate dehydrogenase in wistar rats.姜黄素对西维因诱导的Wistar大鼠乳酸脱氢酶活性紊乱的改善作用研究。
Saudi J Biol Sci. 2018 Dec;25(8):1585-1592. doi: 10.1016/j.sjbs.2016.03.002. Epub 2016 Mar 11.
5
Carbofuran hampers oligodendrocytes development leading to impaired myelination in the hippocampus of rat brain.呋喃丹会阻碍少突胶质细胞的发育，导致大鼠大脑海马区髓鞘形成受损。
Neurotoxicology. 2019 Jan;70:161-179. doi: 10.1016/j.neuro.2018.11.007. Epub 2018 Nov 22.
6
PAI-1 Exacerbates White Adipose Tissue Dysfunction and Metabolic Dysregulation in High Fat Diet-Induced Obesity.纤溶酶原激活物抑制剂-1加剧高脂饮食诱导肥胖中的白色脂肪组织功能障碍和代谢失调。
Front Pharmacol. 2018 Sep 26;9:1087. doi: 10.3389/fphar.2018.01087. eCollection 2018.
7
Oxidative stress, aging, and diseases.氧化应激、衰老和疾病。
Clin Interv Aging. 2018 Apr 26;13:757-772. doi: 10.2147/CIA.S158513. eCollection 2018.
8
An Interplay between Senescence, Apoptosis and Autophagy in Glioblastoma Multiforme-Role in Pathogenesis and Therapeutic Perspective.衰老、细胞凋亡与自噬在多形性胶质母细胞瘤中的相互作用——在发病机制和治疗观点中的作用。
Int J Mol Sci. 2018 Mar 17;19(3):889. doi: 10.3390/ijms19030889.
9
Inhibition of the transforming growth factor-β/SMAD cascade mitigates the anti-neurogenic effects of the carbamate pesticide carbofuran.抑制转化生长因子-β/SMAD级联反应可减轻氨基甲酸酯类农药克百威的抗神经生成作用。
J Biol Chem. 2017 Nov 24;292(47):19423-19440. doi: 10.1074/jbc.M117.798074. Epub 2017 Oct 5.
10
Vitamin C acts as a hepatoprotectant in carbofuran treated rat liver slices .维生素C在被呋喃丹处理过的大鼠肝脏切片中起到肝脏保护剂的作用。
Toxicol Rep. 2017 Jun 9;4:265-273. doi: 10.1016/j.toxrep.2017.06.001. eCollection 2017.