Department of Biochemistry, AIIMS, Mangalagiri, Andhra Pradesh 522503, India.
Neonatology, JIPMER, Puducherry 605006, India.
J Trop Pediatr. 2021 Jan 29;67(1). doi: 10.1093/tropej/fmaa108.
Intrauterine growth restriction (IUGR) is associated with faltered growth and development later in life. Alteration in DNA methylation may occur among IUGR babies and it can have bearing on the outcome.
To compare the DNA methylation in the cord blood among IUGR and appropriate for gestational age (AGA) babies and find it is association with their neurodevelopmental outcome at 18 months of age.
Genomic DNA methylation among 40 IUGR and equal number of AGA neonates was estimated by using 5-mC ELISA kit in the cord blood. Infants were assessed at birth and their anthropometric measurements were taken. They were regularly followed up and assessed for neurodevelopment outcome till 18 months of age using DASII (Developmental Assessment Scale for Indian Infants). DNA methylation was correlated with neurodevelopmental outcome. Numbers and percentages were used for categorical data. Mean and SD were used for continuous variables. The significant mean difference between IUGR and AGA was determined by independent Student t-test. To study the association between the DNA methylation and outcome, Spearman correlation was used. A p < 0.05 was considered as statistically significant.
Significant difference in DNA methylation was observed between IUGR and AGA infants (IUGR: 3.12 ± 1.24; AGA: 4.40 ± 2.03; p < 0.001). Anthropometry (weight, length and head circumference) at birth was significantly decreased among IUGR infants. Hospital stay was significantly longer for IUGR infants. Motor (IUGR: 89.98 ± 18.77; AGA: 101.75 ± 9.62; p < 0.001), and mental (IUGR: 90.81 ± 11.13; AGA: 105.71 ± 7.20; p < 0.001) scores were significantly decreased among IUGR compared with AGA neonates at 18 months of follow-up. Global DNA methylation had a significant positive correlation with mental score but not with motor developmental score.
IUGR babies had lower motor and mental score compared with AGA babies. Cord blood global DNA methylation significantly correlated with mental development score but not with motor development at 18 months of age.
宫内生长受限（IUGR）与生命后期生长和发育迟缓有关。IUGR 婴儿的 DNA 甲基化可能发生改变，这可能对其结果产生影响。
比较 IUGR 和适合胎龄（AGA）婴儿脐带血中的 DNA 甲基化，并发现其与 18 个月时的神经发育结局有关。
使用 5-mC ELISA 试剂盒检测 40 例 IUGR 和同等数量 AGA 新生儿脐带血中的基因组 DNA 甲基化。在出生时评估婴儿，并测量其人体测量学指标。定期随访并在 18 个月时使用 DASII（印度婴儿发育评估量表）评估神经发育结局。将 DNA 甲基化与神经发育结局相关联。使用数字和百分比表示分类数据。使用均值和标准差表示连续变量。通过独立学生 t 检验确定 IUGR 和 AGA 之间的显著平均差异。为了研究 DNA 甲基化与结局之间的关系，使用 Spearman 相关性。p<0.05 被认为具有统计学意义。
IUGR 和 AGA 婴儿的 DNA 甲基化存在显著差异（IUGR：3.12±1.24；AGA：4.40±2.03；p<0.001）。IUGR 婴儿出生时的人体测量（体重、长度和头围）明显减少。IUGR 婴儿的住院时间明显延长。运动（IUGR：89.98±18.77；AGA：101.75±9.62；p<0.001）和精神（IUGR：90.81±11.13；AGA：105.71±7.20；p<0.001）评分在 18 个月随访时明显低于 AGA 新生儿。全球 DNA 甲基化与精神评分呈显著正相关，但与运动发育评分无关。
与 AGA 婴儿相比，IUGR 婴儿的运动和精神评分较低。脐带血的全基因组 DNA 甲基化与 18 个月时的精神发育评分显著相关，但与运动发育评分无关。
