Yang Sen, Qiao Lina, Shi Jing, Xie Liang, Liu Yang, Xiong Ying, Liu Hanmin
Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, China.
Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, China.
Front Pediatr. 2021 Feb 16;9:632573. doi: 10.3389/fped.2021.632573. eCollection 2021.
There are mutual influences between intestine and lung, that propose a concept of the gut-lung axis, but the mechanism is still unclear. Microbial colonization in early life plays an important role in regulating intestinal and lung function. In order to explore the characteristics of early microbiota on the gut-lung axis, we studied the correlation between intestinal and pharyngeal microbiota on day 1 and day 28 after birth in premature neonates. Thirteen neonates born at 26-32 weeks gestational age (GA) hospitalized at the neonatal intensive care unit (NICU) of the West China Second Hospital of Sichuan University were enrolled in this study. Stool samples and pharyngeal swabs samples were collected from each neonate on the first day (T1) and the 28th day (T28) after birth. Total bacterial DNA was extracted and sequenced using the Illumina MiSeq Sequencing System based on the V3-V4 hyper-variable regions of the 16S rRNA gene. Based on the sequencing results, the composition of the intestinal and pharyngeal microbiota was compared and analyzed. At T1, the difference in microbial composition between intestine and pharynx was not statistically significant. The intestinal microbiota was mainly composed of , etc. The pharyngeal microbiota was mainly composed of , etc. and were detected in both intestine and pharynx. At T28, there was a significant difference in microbial composition between intestine and pharynx ( < 0.001). The intestinal microbiota was mainly composed of , etc. Pharyngeal microbiota was mainly composed of , etc. was detected in both intestine and pharynx. The intestine and pharynx of premature neonates have a unique microbial composition, and share some common microbiota. Whether these microbiotas play a role in the mechanism of gut-lung crosstalk needs further study.
肠道与肺之间存在相互影响，由此提出了肠-肺轴的概念，但具体机制仍不清楚。生命早期的微生物定植在调节肠道和肺功能方面发挥着重要作用。为了探究早期微生物群在肠-肺轴上的特征，我们研究了早产儿出生后第1天和第28天肠道和咽部微生物群之间的相关性。本研究纳入了四川大学华西第二医院新生儿重症监护病房（NICU）收治的13例胎龄为26-32周的早产儿。在出生后的第1天（T1）和第28天（T28）采集每个新生儿的粪便样本和咽拭子样本。基于16S rRNA基因的V3-V4高变区，使用Illumina MiSeq测序系统提取总细菌DNA并进行测序。根据测序结果，对肠道和咽部微生物群的组成进行比较和分析。在T1时，肠道和咽部微生物组成的差异无统计学意义。肠道微生物群主要由 等组成。咽部微生物群主要由 等组成。肠道和咽部均检测到 和 。在T28时，肠道和咽部微生物组成存在显著差异（<0.001）。肠道微生物群主要由 等组成。咽部微生物群主要由 等组成。肠道和咽部均检测到 。早产儿的肠道和咽部具有独特的微生物组成，并共享一些共同的微生物群。这些微生物群是否在肠-肺串扰机制中发挥作用需要进一步研究。
