Cai Fei-Fan, Heid Susanne, Boccaccini Aldo R
Department of Materials Science and Engineering, Institute of Biomaterials, University of Erlangen-Nuremberg, Erlangen, Germany.
J Biomed Mater Res B Appl Biomater. 2021 Aug;109(8):1090-1104. doi: 10.1002/jbm.b.34771. Epub 2020 Dec 5.
The concept of adding inorganic fillers into hydrogels to form hydrogel nanocomposites often provides advantageous properties which can be exploited for successful 3D biofabrication. In this study, a new composite hydrogel combining oxidized alginate-gelatin (ADA-GEL) hydrogel and Laponite® nanoclay as inorganic nanofiller was successfully developed and characterized. The results showed that the addition of 0.5% (wt/vol) Laponite® nanoplatelets improved the printability of ADA-GEL hydrogels enabling the fabrication of detailed structures since a low effect of material spreading and reduced tendency to pore closure appeared. Furthermore, a comparison of different needle types (cylindrical and conical; same inner diameter of 250 μm) in filament fusion test showed that the pattern dispensed by cylindrical tip has enhanced printing accuracy and pattern fidelity when compared with the pattern from conical tip. A glass flip test determined a processing window of 1-2 h after composite ink preparation. Overall, Laponite® /ADA-GEL hydrogel composites are confirmed as promising inks for 3D bioprinting.
在水凝胶中添加无机填料以形成水凝胶纳米复合材料的概念通常会提供有利的性能，这些性能可用于成功的3D生物制造。在本研究中，成功开发并表征了一种将氧化海藻酸盐 - 明胶（ADA - GEL）水凝胶与Laponite®纳米粘土作为无机纳米填料相结合的新型复合水凝胶。结果表明，添加0.5%（重量/体积）的Laponite®纳米片提高了ADA - GEL水凝胶的可打印性，由于材料铺展效应低且孔隙闭合趋势降低，从而能够制造出精细的结构。此外，在长丝融合测试中对不同针型（圆柱形和圆锥形；相同内径250μm）的比较表明，与圆锥形尖端的图案相比，圆柱形尖端喷出的图案具有更高的打印精度和图案保真度。玻璃翻转测试确定了复合墨水制备后1 - 2小时的加工窗口。总体而言，Laponite® /ADA - GEL水凝胶复合材料被确认为用于3D生物打印的有前景的墨水。
