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体外甲状腺细针穿刺抽吸术作为基质辅助激光解吸电离质谱成像蛋白质组学研究的替代方法：患者内比较

Ex vivo thyroid fine needle aspirations as an alternative for MALDI-MSI proteomic investigation: intra-patient comparison.

作者信息

Piga Isabella, Capitoli Giulia, Clerici Francesca, Mahajneh Allia, Brambilla Virginia, Smith Andrew, Leni Davide, L'Imperio Vincenzo, Galimberti Stefania, Pagni Fabio, Magni Fulvio

机构信息

Proteomics and Metabolomics Unit, School of Medicine and Surgery, University of Milano - Bicocca, 20854, Vedano al Lambro, Italy.

Bicocca Bioinformatics Biostatistics and Bioimaging B4 Center, School of Medicine and Surgery, University of Milano - Bicocca, 20900, Monza, Italy.

出版信息

Anal Bioanal Chem. 2021 Feb;413(5):1259-1266. doi: 10.1007/s00216-020-03088-4. Epub 2020 Dec 5.

DOI:10.1007/s00216-020-03088-4
PMID:33277997
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7892726/
Abstract

Fine needle aspiration (FNA) is the reference standard for the diagnosis of thyroid nodules. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been successfully used to discriminate the proteomic profiles of benign and malignant thyroid FNAs within the scope of providing support to pathologists for the classification of morphologically borderline cases. However, real FNAs provide a limited amount of material due to sample collection restrictions. Ex vivo FNAs could represent a valuable alternative, increasing sample size and the power of statistical conclusions. In this study, we compared the real and ex vivo MALDI-MSI proteomic profiles, extracted from thyrocyte containing regions of interest, of 13 patients in order to verify their similarity. Statistical analysis demonstrated the mass spectra similarity of the proteomic profiles by performing intra-patient comparison, using statistical similarity systems. In conclusion, these results show that post-surgical FNAs represent a possible alternative source of material for MALDI-MSI proteomic investigations in instances where pre-surgical samples are unavailable or the number of cells is scarce.

摘要

细针穿刺抽吸活检（FNA）是诊断甲状腺结节的参考标准。基质辅助激光解吸/电离质谱成像（MALDI-MSI）已成功用于区分良性和恶性甲状腺FNA的蛋白质组学特征，为病理学家对形态学上临界病例的分类提供支持。然而，由于样本采集限制，实际的FNA提供的材料数量有限。体外FNA可能是一种有价值的替代方法，可增加样本量并提高统计结论的说服力。在本研究中，我们比较了13例患者从含甲状腺细胞的感兴趣区域提取的实际和体外MALDI-MSI蛋白质组学特征，以验证它们的相似性。统计分析通过使用统计相似性系统进行患者内比较，证明了蛋白质组学特征的质谱相似性。总之，这些结果表明，在无法获得手术前样本或细胞数量稀少的情况下，手术后FNA是MALDI-MSI蛋白质组学研究的一种可能的替代材料来源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc3/7892726/327893196086/216_2020_3088_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc3/7892726/60555b19f0af/216_2020_3088_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc3/7892726/98d6283c47d9/216_2020_3088_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc3/7892726/8da5f97f66be/216_2020_3088_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc3/7892726/327893196086/216_2020_3088_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc3/7892726/60555b19f0af/216_2020_3088_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc3/7892726/98d6283c47d9/216_2020_3088_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc3/7892726/8da5f97f66be/216_2020_3088_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cdc3/7892726/327893196086/216_2020_3088_Fig4_HTML.jpg

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Proc Natl Acad Sci U S A. 2019 Oct 22;116(43):21401-21408. doi: 10.1073/pnas.1911333116. Epub 2019 Oct 7.
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MALDI-MSI as a Complementary Diagnostic Tool in Cytopathology: A Pilot Study for the Characterization of Thyroid Nodules.基质辅助激光解吸电离质谱成像作为细胞病理学中的一种辅助诊断工具：甲状腺结节特征的初步研究
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