PhD Scholar, School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animals Sciences University, Ludhiana, India.
Master`s student, School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India.
FEMS Microbiol Lett. 2020 Dec 22;367(23). doi: 10.1093/femsle/fnaa201.
The aim of this study was to illustrate the relative pervasiveness of Borderline Oxacillin Resistant Staphylococcus aureus (BORSA) and Methicillin-Resistant Staphylococcus aureus (MRSA) in community and food of animal origin and their relationship with other genetic determinants. Staphylococcus aureus isolates were subjected to E-test using the antibiotics: oxacillin, ceftriaxone, cotrimoxazole, vancomycin, genotypic tests for the genes mecA, vanA, blaZ, pvl gene and SCCmec typing. The prevalence of S. aureus (MRSA) in the food of animal origin and community settings was 21% (1.8% MRSA) and 21.9% (7.4% MRSA), respectively. SCCmec type V was prevalent among the food of animal origin, while SCCmec type IVa among the community isolates. The likelihood of MRSA presence among community isolates was three times more than in isolates from chicken and milk samples. Likewise, the likelihood of detecting pvl positive MRSA (pvl+MRSA) isolates was 4-fold higher in the community setting than in the food of animal origin. The mecA negative BORSA (mecA-BORSA) was a frequently observed phenotype among S. aureus isolates. Also, co-detection of pvl and cotrimoxazol resistance was reported in this study although there was no noteworthy correlation of cotrimoxazol resistance with the type of sample. Isolates from milk and community settings exhibit higher minimum inhibitory concentration to vancomycin (Vancomycin MIC creep, 2-4 µg/mL).
Current study provides the information on the statistical relationship between the genetic determinants of S. aureus with respect to sample type, and additionally the correlation that exists between the pvl and MRSA, pvl and cotrimoxazol resistance, vancomycin MIC and MRSA/Methicillin-Susceptible S. aureus (MSSA).
本研究旨在阐明社区和动物源食品中边缘型耐苯唑西林金黄色葡萄球菌（BORSA）和耐甲氧西林金黄色葡萄球菌（MRSA）的相对普遍性及其与其他遗传决定因素的关系。使用抗生素：苯唑西林、头孢曲松、复方新诺明、万古霉素对金黄色葡萄球菌分离株进行 E 试验，进行 mecA、vanA、blaZ、pvl 基因和 SCCmec 型基因检测。动物源性食品和社区环境中金黄色葡萄球菌（MRSA）的流行率分别为 21%（1.8%MRSA）和 21.9%（7.4%MRSA）。SCCmec 型 V 多见于动物源性食品，而 SCCmec 型 IVa 多见于社区分离株。社区分离株中 MRSA 存在的可能性是鸡和牛奶样本的 3 倍。同样，社区环境中检测到 pvl 阳性 MRSA（pvl+MRSA）的可能性是动物源性食品的 4 倍。mecA 阴性 BORSA（mecA-BORSA）是金黄色葡萄球菌分离株中经常观察到的表型。此外，本研究还报道了 pvl 和复方新诺明耐药的共同检测，尽管复方新诺明耐药与样本类型之间没有明显相关性。来自牛奶和社区环境的分离株对万古霉素的最小抑菌浓度（万古霉素 MIC 攀升，2-4µg/mL）更高。
本研究提供了关于金黄色葡萄球菌遗传决定因素与样本类型之间的统计关系的信息，此外还存在 pvl 与 MRSA、pvl 与复方新诺明耐药、万古霉素 MIC 与 MRSA/甲氧西林敏感金黄色葡萄球菌（MSSA）之间的相关性。
