益生菌双歧杆菌对与牙周病相关的病原体生物膜的拮抗作用。
Antagonist effect of probiotic bifidobacteria on biofilms of pathogens associated with periodontal disease.
机构信息
São Paulo State University (UNESP), School of Dentistry, Araçatuba, Department of Preventive and Restorative Dentistry, Araçatuba, São Paulo, Brazil.
Universidad Nacional Autónoma de México, Faculty of Dentistry, Department of Postgraduate Studies and Research, Ciudad Universitaria, México D.F., Mexico.
出版信息
Microb Pathog. 2021 Jan;150:104657. doi: 10.1016/j.micpath.2020.104657. Epub 2020 Dec 2.
The in vitro antagonist growth effect of bifidobacteria were evaluated on periodontal bacteria. Bifidobacterium longum, Bifidobacterium lactis and Bifidobacterium infantis biofilms were grown in single, double or triple combinations with putative periodontal pathogens P. gingivalis and F. nucleatum or beneficial bacteria S. oralis for 24, 72 and 168 h and the total counts were analyzed by checkerboard DNA-DNA hybridization. The results showed that B. infantis and B. lactis, as single species, demonstrated the best antagonist effect on F. nucleatum and P. gingivalis and no influence on S. oralis growth at 168 h. All the double combinations of bifidobacteria tested demonstrated an inhibitory effect on F. nucleatum (72 h) and P. gingivalis (168 h) and did not affect S. oralis counts at any time. In conclusion, B. lactis and B. infantis alone or in double combinations have antagonist effect on periodontopathogens biofilms, at different time points, and minimal influence on S. oralis growth.
双歧杆菌对牙周致病菌的体外拮抗生长作用。将长双歧杆菌、嗜酸乳杆菌和婴儿双歧杆菌生物膜分别与疑似牙周病原菌牙龈卟啉单胞菌和具核梭杆菌或有益菌口腔链球菌以单种、两种或三种组合的方式培养 24、72 和 168 小时，并通过棋盘式 DNA-DNA 杂交分析总计数。结果表明，双歧杆菌属婴儿双歧杆菌和嗜酸乳杆菌作为单一物种，对具核梭杆菌和牙龈卟啉单胞菌表现出最佳的拮抗作用，而对 168 小时的口腔链球菌生长无影响。所有测试的双歧杆菌双组合在 72 小时时均显示出对具核梭杆菌的抑制作用（72 小时）和牙龈卟啉单胞菌（168 小时），并且在任何时间都不影响口腔链球菌的计数。总之，嗜酸乳杆菌和婴儿双歧杆菌单独或双组合在不同时间点对牙周致病菌生物膜具有拮抗作用，对口腔链球菌的生长影响最小。
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