Department of Biotechnology and Microbiology, Institute of Food Science Research (CIAL), CSIC-UAM, Madrid, Spain.
Department of Health and Genomics, Center for Advanced Research in Public Health, FISABIO Foundation, Valencia, Spain.
J Periodontol. 2019 Nov;90(11):1346-1355. doi: 10.1002/JPER.18-0751. Epub 2019 Jun 12.
Periodontal diseases are of high prevalence globally and are characterized by an exacerbated inflammatory response which leads to oral tissue destruction. The use of probiotics is widely extended in the case of gastrointestinal disorders; however, their use in microbial-origin oral diseases is still preliminary.
We used quantitative polymerase chain reaction to determine the levels of the oral bacterium Streptococcus dentisani 7746 in the tongue, saliva, supragingival, and subgingival plaque. We explore the potential benefits of this probiotic by measuring inhibition of the periodontal pathogens Porphyromonas gingivalis and Fusobacterium nucleatum growth and attachment to human gingival fibroblasts. In addition, its anti-inflammatory activity against cytokines secretion induced by these pathogens was determined in an in vitro model by enzyme-linked immunosorbent assay.
We report that S. dentisani is found at high levels in the gingival crevice. Data show a strong inhibitory action of S. dentisani supernatant against the periodontal pathogens in pure culture. S. dentisani attached to gingival cells in vitro, inhibiting periodontal pathogens by competition, adherence, and displacement mechanisms. Finally, in a simple in vitro model, the oral probiotic strongly increased the secretion of the anti-inflammatory cytokine IL-10 after incubations with P. gingivalis and F. nucleatum, as well as significantly reduced the expression of interferon-γ induced by F. nucleatum.
Altogether, these results highlight the potential of S. dentisani as adjuvant therapy in the management of periodontal diseases, whose efficacy will need to be tested in clinical studies.
牙周病在全球范围内发病率很高，其特征是炎症反应加剧，导致口腔组织破坏。益生菌的使用在胃肠道疾病中广泛扩展；然而，它们在微生物来源的口腔疾病中的应用仍处于初步阶段。
我们使用定量聚合酶链反应来确定舌、唾液、龈上和龈下菌斑中口腔细菌 Streptococcus dentisani 7746 的水平。我们通过测量对牙周病原体牙龈卟啉单胞菌和具核梭杆菌生长和附着到人牙龈成纤维细胞的抑制作用来探索这种益生菌的潜在益处。此外，通过酶联免疫吸附试验在体外模型中测定其对这些病原体诱导的细胞因子分泌的抗炎活性。
我们报告说 S. dentisani 在牙龈沟中水平很高。数据显示 S. dentisani 上清液对纯培养中的牙周病原体具有强烈的抑制作用。S. dentisani 附着在体外的牙龈细胞上，通过竞争、粘附和置换机制抑制牙周病原体。最后，在一个简单的体外模型中，口腔益生菌在与牙龈卟啉单胞菌和具核梭杆菌孵育后，强烈增加抗炎细胞因子 IL-10 的分泌，并显著降低具核梭杆菌诱导的干扰素-γ的表达。
总之，这些结果强调了 S. dentisani 作为牙周病管理辅助治疗的潜力，其疗效需要在临床研究中进行测试。
