The Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada; Department of Medicine, McGill University, Montreal, Quebec, Canada.
The Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada.
Gene. 2021 Feb 5;768:145332. doi: 10.1016/j.gene.2020.145332. Epub 2020 Dec 3.
Repetitive short interspersed elements B2 (SINE B2) have been shown to possess two promoters: polymerase III promoter for producing short B2-S RNAs and polymerase II promoter for driving the expression of long non-coding RNA (B2-AS lncRNAs). Using a B2-antisense (B2-AS) transcript sequence from the SINE B2 resident in mitochondrial translocator protein gene (Tspo) locus, we constructed a B2-AS specific RNA library and identified 96,862 sequences encoding potential B2-mediated lncRNAs, of which 55,592 lncRNAs with more than 390 nt in length possess a feature of potential genomic locus-specific effect. In addition, small RNA-Northern hybridization showed that the new B2-AS lncRNAs are constantly degraded by the Dicer1 enzyme, a finding further confirmed by in vitro Dicer1 enzyme digestion. B2-AS lncRNAs regulate the expression of target genes in a different fashion than B2-S RNAs. Genome-wide cross-comparison with mRNA mapping showed a total of 904 mRNA loci directly targeted by B2-AS lncRNAs, suggesting a locus-specific effect of the B2-AS lncRNAs and a general effect of B2-S RNAs.
重复短散布元件 B2（SINE B2）已被证明具有两个启动子：产生短 B2-S RNAs 的聚合酶 III 启动子和驱动长非编码 RNA（B2-AS lncRNAs）表达的聚合酶 II 启动子。我们使用来自线粒体转位蛋白基因（Tspo）基因座中驻留的 SINE B2 的 B2-反义（B2-AS）转录本序列，构建了 B2-AS 特异性 RNA 文库，鉴定了 96,862 个编码潜在 B2 介导的 lncRNAs 的序列，其中 55,592 个长度超过 390 nt 的 lncRNAs 具有潜在基因组基因座特异性效应的特征。此外，小 RNA-Northern 杂交显示新的 B2-AS lncRNAs 不断被 Dicer1 酶降解，体外 Dicer1 酶消化实验进一步证实了这一发现。B2-AS lncRNAs 以不同于 B2-S RNAs 的方式调节靶基因的表达。与 mRNA 映射的全基因组比较显示，共有 904 个 mRNA 基因座直接受到 B2-AS lncRNAs 的靶向作用，这表明 B2-AS lncRNAs 具有基因座特异性效应和 B2-S RNAs 的一般效应。
