School Life and Pharmaceutical Sciences, Engineering Research Center for Utilization of Tropical Polysaccharide Resources, Ministry of Education, Hainan University, Haikou 570228, China.
School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China.
Carbohydr Polym. 2021 Feb 1;253:117197. doi: 10.1016/j.carbpol.2020.117197. Epub 2020 Oct 10.
Phomopsis liquidambari is a classical endophytic fungus with great application potential in ecology and agriculture; however, studies on its exopolysaccharides are lacking. Here, we aimed to evaluate the structure and bioactivity of PLN-1, an exopolysaccharide derived from the P. liquidambari NJUSTb1 strain. The structure was elucidated by chromatography/spectral methods and hydrolyzation. Immunomodulation, moisture absorption, and retention properties were investigated after sulfation and carboxymethylation modification. Results showed that PLN-1 contained a linear repeating unit of →[4)-α-d-Glcp-(1→6)-α-d-Glcp-(1→4)-α-d-Glcp-(1→4)-α-d-Glcp-(1→], with a molecular weight of 343 kDa. The degrees of substitution of sulfated polysaccharide (S-PLN-1) and carboxymethylated polysaccharide (C-PLN-1) were 1.228 and 0.903, respectively. S-PLN-1 showed stronger moisture absorption and retention properties than PLN (crude EPS), C-PLN1, and PLN-1. Furthermore, PLN, S-PLN-1, and C-PLN-1 stimulated the proliferation of RAW 264.7 cells with no cytotoxicity. The elucidation of PLN-1 in this study paves the way for future applications.
霍氏拟茎点霉是一种经典的内生真菌，在生态学和农业领域具有巨大的应用潜力；然而，关于其胞外多糖的研究还很少。在这里，我们旨在评估来源于霍氏拟茎点霉 NJUSTb1 菌株的胞外多糖 PLN-1 的结构和生物活性。通过色谱/光谱方法和水解对其结构进行了阐明。经过硫酸化和羧甲基化修饰后，研究了其免疫调节、吸湿和保湿性能。结果表明，PLN-1 含有一个线性重复单元→[4）-α-d-Glcp-（1→6）-α-d-Glcp-（1→4）-α-d-Glcp-（1→4）-α-d-Glcp-（1→]，分子量为 343 kDa。硫酸化多糖（S-PLN-1）和羧甲基化多糖（C-PLN-1）的取代度分别为 1.228 和 0.903。S-PLN-1 具有比 PLN（粗 EPS）、C-PLN1 和 PLN-1 更强的吸湿和保湿性能。此外，PLN、S-PLN-1 和 C-PLN-1 对 RAW 264.7 细胞的增殖具有刺激作用，且没有细胞毒性。本研究中 PLN-1 的阐明为其未来的应用奠定了基础。
