State Key Laboratory of Food Nutrition and Safety, School of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin, 300457, China; College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, China.
Shanghai Engineering Research Center of Food Safety, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Carbohydr Polym. 2021 Jun 15;262:117945. doi: 10.1016/j.carbpol.2021.117945. Epub 2021 Mar 15.
Alkaline extracted endopolysaccharides (MPS) from Monascus purpureus (Hong Qu) mycelium were successfully separated into four sub-fractions, namely MPS-1 (18.0 %), MPS-2 (27.1 %), MPS-3 (12.6 %) and MPS-4 (14.7 %), by DEAE-Cellulose column chromatography. By combining monosaccharide composition analysis, methylation analysis and 1D & 2D NMR, the structure of sub-fractions was systematically characterized. Both MPS-1 and MPS-2 were comprised of mannose, glucose and galactose in the molar ratio of 1.5:1.6:1.0 and 10.6:1.0:13.8, respectively. The backbone of them both consisted of 2-α-Manp with several different branched chains. However, MPS-1 contained glucose based sugar residues such as 3-Glcp and 4-Glcp which were not shown on MPS-2. The proposed structures of MPS-3 and MPS-4 were not obtained due to the fairly complex molecular structure and relatively low yield. Moreover, based on the RAW 264.7 cells model, MPS-2 could significantly promote cytokines secretion including IL-6, TNF-α, and IL-10 and improve expression levels of the related mRNA.
从红曲菌（Monascus purpureus）菌丝体中提取的碱性胞外多糖（MPS），通过 DEAE-纤维素柱层析成功分离成四个亚组分，分别为 MPS-1（18.0%）、MPS-2（27.1%）、MPS-3（12.6%）和 MPS-4（14.7%）。通过单糖组成分析、甲基化分析和 1D 和 2D NMR，系统地表征了亚组分的结构。MPS-1 和 MPS-2 均由甘露糖、葡萄糖和半乳糖组成，摩尔比分别为 1.5:1.6:1.0 和 10.6:1.0:13.8。它们的骨架均由 2-α-Manp 组成，具有几个不同的支链。然而，MPS-1 含有葡萄糖基糖残基，如 3-Glcp 和 4-Glcp，而 MPS-2 则没有。由于分子结构相当复杂，产率相对较低，因此未能获得 MPS-3 和 MPS-4 的推测结构。此外，基于 RAW 264.7 细胞模型，MPS-2 可显著促进细胞因子的分泌，包括 IL-6、TNF-α 和 IL-10，并提高相关 mRNA 的表达水平。
