Hori Atsushi, Ishida Fumihiro, Nakazawa Hideyuki, Yamaura Makoto, Morita Sunao, Uehara Takeshi, Honda Takayuki, Hidaka Hiroya
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, Matsumoto, Japan; Department of Clinical Laboratory Medicine, Juntendo University Graduate School of Medicine, Bunkyo-ku, Japan; Center for Genomic and Regenerative Medicine, Juntendo University Graduate School of Medicine, Bunkyo-ku, Japan.
Department of Biomedical Laboratory Sciences, School of Health Sciences, Shinshu University School of Medicine, Matsumoto, Japan; Division of Hematology, Department of Internal Medicine, Shinshu University School of Medicine, Matsumoto, Japan.
Clin Chim Acta. 2021 Mar;514:29-33. doi: 10.1016/j.cca.2020.11.024. Epub 2020 Dec 3.
Sphingomyelin (SM) plays key roles in regulating cell membrane fluidity and in intracellular signal transduction. However, little is known as to whether alterations in SM concentration or SM species distribution are linked pathological conditions. The present study examined SM concentrations and species profiles in serum taken from patients with hematologic malignancies. Serum was collected from normal subjects and from patients with B-cell lymphoma, myelodysplastic syndrome (MDS), acute myeloid leukemia (AML) and acute lymphatic leukemia/ lymphoblastic lymphoma (ALL/LBL). Serum SM species distribution was analyzed using electrospray ionization mass spectrometry/ mass spectrometry (ESI MS/MS). Serum lipids concentration were measured using enzymatic assays. Normal and hematologic malignancy sera were similar in terms of total serum SM and phosphatidylcholine (PC) concentrations and SM/PC ratio. However, all hematologic malignancy sera had lower levels of SM species containing saturated odd chain fatty acids (OCFAs) in the side chain compared to normal serum. In addition, the proportion of SM species with saturated (C20 and C22) and mono unsaturated fatty acids (C18, C20, C22) were lower in MDS patient serum compared to normal serum. The present study revealed that the serum SM species profile in patients with hematologic malignancies differed from that of normal subjects despite total serum SM and PC concentrations and SM/PC ratios being similar between the various cancer groups and the normal group.
鞘磷脂（SM）在调节细胞膜流动性和细胞内信号转导中起关键作用。然而，关于SM浓度或SM种类分布的改变是否与病理状况相关，目前所知甚少。本研究检测了血液系统恶性肿瘤患者血清中的SM浓度和种类谱。从正常受试者以及B细胞淋巴瘤、骨髓增生异常综合征（MDS）、急性髓系白血病（AML）和急性淋巴细胞白血病/淋巴细胞淋巴瘤（ALL/LBL）患者中采集血清。使用电喷雾电离质谱/质谱（ESI MS/MS）分析血清SM种类分布。采用酶法测定血清脂质浓度。正常血清和血液系统恶性肿瘤血清在总血清SM、磷脂酰胆碱（PC）浓度以及SM/PC比值方面相似。然而，与正常血清相比，所有血液系统恶性肿瘤血清中侧链含有饱和奇数链脂肪酸（OCFAs）的SM种类水平较低。此外，与正常血清相比，MDS患者血清中含有饱和（C20和C22）和单不饱和脂肪酸（C18、C20、C22）的SM种类比例较低。本研究表明，尽管各癌症组与正常组之间的总血清SM、PC浓度以及SM/PC比值相似，但血液系统恶性肿瘤患者的血清SM种类谱与正常受试者不同。
