Department of Chemistry, College of Science, King Khalid University, Abha, Saudi Arabia; Department of Chemistry, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt.
Department of Chemistry, Faculty of Science, Jazan University, Saudi Arabia.
Int J Pharm. 2021 Jan 25;593:120143. doi: 10.1016/j.ijpharm.2020.120143. Epub 2020 Dec 3.
The development of a scaffold matrix that can inhibit bacterial infection and promote wound healing simultaneously is an essential demand to improve the health care system. Hydroxyapatite (HAP) doped with different concentrations of silver ions (Ag) were incorporated into electrospun nanofibrous scaffolds of polycaprolactone (PCL) using the electrospinning technique. The formed phase was identified using XRD, while the morphological and roughness behavior were investigated using FESEM. It was shown that scaffolds were configured in randomly distributed nanofibers with diameters around of 0.19-0.40, 0.31-0.54, 1.36, 0.122-0.429 μm for 0.0Ag-HAP@PCL, 0.2Ag-HAP@PCL, 0.6Ag-HAP@PCL, and 0.8Ag-HAP@PCL, respectively. Moreover, the maximum roughness peak height increased significantly from 179 to 284 nm, with the lowest and highest contributions of Ag. The mechanical properties were examined and displayed that the tensile strength increased from 3.11 ± 0.21 MPa to its highest value at 3.57 ± 0.31 MPa for 0.4Ag-HAP@PCL. On the other hand, the cell viability also was enhanced with the addition of Ag and improved from 97.1 ± 4.6% to be around 102.3 ± 3.1% at the highest contribution of Ag. The antibacterial activity was determined, and the highest imbibition zones were achieved at the highest Ag dopant to be 12.5 ± 1.1 mm and 11.4 ± 1.5 mm against E. coli and S. aureus. The in vitro cell proliferation was observed through human fibroblasts cell lone (HFB4) and illustrated that cells were able to grow and spread not only on the fibers' surface but also, they were spreading and adhered through the deep pores.
开发一种既能抑制细菌感染又能促进伤口愈合的支架基质，是改善医疗保健系统的重要需求。采用静电纺丝技术，将不同浓度银离子（Ag）掺杂的羟基磷灰石（HAP）掺入聚己内酯（PCL）的电纺纳米纤维支架中。通过 XRD 鉴定形成的相，通过 FESEM 研究形态和粗糙度行为。结果表明，支架由直径约为 0.19-0.40、0.31-0.54、1.36、0.122-0.429μm 的随机分布纳米纤维组成，分别为 0.0Ag-HAP@PCL、0.2Ag-HAP@PCL、0.6Ag-HAP@PCL 和 0.8Ag-HAP@PCL。此外，最大粗糙度峰值高度从 179nm 显著增加到 284nm，Ag 的贡献最低和最高。还测试了力学性能，结果表明，拉伸强度从 3.11±0.21MPa 增加到 0.4Ag-HAP@PCL 的最高值 3.57±0.31MPa。另一方面，随着 Ag 的加入，细胞活力也得到了提高，从 97.1±4.6%提高到最高 Ag 贡献时的 102.3±3.1%。测定了抗菌活性，在最高 Ag 掺杂量时达到了最大的吸液区，分别为 12.5±1.1mm 和 11.4±1.5mm，分别针对大肠杆菌和金黄色葡萄球菌。通过人成纤维细胞系（HFB4）观察了体外细胞增殖，并表明细胞不仅能够在纤维表面生长和扩散，而且还能够通过深孔扩散和粘附。
