一株链黑菌素型生物碱突变株 CGMCC 4.1223Δ 的新型结构。

A novel streptonigrin type alkaloid from the CGMCC 4.1223 mutant Δ.

机构信息

State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory on Metabolic & Developmental Sciences, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, China.

School of Medicine, Zhejiang University, Hangzhou, China.

出版信息

Nat Prod Res. 2022 Jul;36(13):3337-3345. doi: 10.1080/14786419.2020.1856840. Epub 2020 Dec 7.

DOI:10.1080/14786419.2020.1856840

PMID:33280413

Abstract

Streptonigrin (STN) is a highly functionalized aminoquinone alkaloid with broad and potent antitumor activities. Previously, the biosynthetic gene cluster of STN was identified in CGMCC 4.1223, revealing an α/β-hydrolase (StnA) and a methyltransferase (StnQ2). In this work, a double mutant Δ was constructed by genetic manipulation and produced a novel derivative of STN, named as streptonigramide. Structure of streptonigramide was established by spectroscopic analyses. Its biosynthetic pathway has been proposed as well.

摘要

斯特普托宁（STN）是一种具有广泛而强大抗肿瘤活性的高度功能化的氨基醌生物碱。此前，STN 的生物合成基因簇在 CGMCC 4.1223 中被鉴定出来，揭示了一种 α/β-水解酶（StnA）和一种甲基转移酶（StnQ2）。在这项工作中，通过遗传操作构建了一个双突变体Δ，并产生了 STN 的一种新型衍生物，命名为 streptonigramide。通过光谱分析确定了 streptonigramide 的结构，并提出了其生物合成途径。