Sun Tiancheng, Hao Sue, Fan Ruiqing, Zhang Jian, Chen Wei, Zhu Ke, Wang Ping, Fang Xikui, Yang Yulin
MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, PR China.
MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, PR China.
Anal Chim Acta. 2021 Jan 15;1142:211-220. doi: 10.1016/j.aca.2020.10.062. Epub 2020 Nov 5.
Developing portable membrane sensors to accurately detect the biomolecule ascorbic acid (AA) is extremely important for food safety and human health. Herein, we successfully design and synthesize a novel cationic metal organic framework (Eu-pbmc, Hpbmc = 2-(pyridine-2-yl)-1H-benzimidazole-5-carboxylic acid) and assemble polyacrylonitrile/Eu-pbmc membrane (PEM) by an in-situ growth strategy. Benefiting from the appreciable loading of Eu-pbmc nanoparticles and high water permeation flux, PEM possesses effective detection for MnO with a limit of detection (LOD) of 17 nM. Utilizing the cationic porous framework, we load MnO into PEM and construct a "on-off-on" system for effective AA detection. The oxidative MnO can be reduced by AA and the resulting turn-on luminescence can reflect the concentration of AA. Compared with pure Eu-pbmc crystals, PEM exhibits improved AA detection performance with LOD of 48 nM and detection time of 1 min via a concise detection operation. The stable membrane sensor realizes an accurate detection in real biological samples, meeting the practical requirement. Moreover, an IMP logic gate is helpful to analyze MnO and AA in water. The proposed novel luminescence platform as well as reasonable "on-off-on" luminescence mode provide a promising method for AA detection.
开发便携式膜传感器以准确检测生物分子抗坏血酸（AA）对食品安全和人类健康极为重要。在此，我们成功设计并合成了一种新型阳离子金属有机框架（Eu-pbmc，Hpbmc = 2-(吡啶-2-基)-1H-苯并咪唑-5-羧酸），并通过原位生长策略组装了聚丙烯腈/Eu-pbmc膜（PEM）。受益于Eu-pbmc纳米颗粒的可观负载量和高水渗透通量，PEM对MnO具有有效的检测能力，检测限（LOD）为17 nM。利用阳离子多孔框架，我们将MnO负载到PEM中，并构建了一个用于有效检测AA的“开-关-开”系统。氧化性的MnO可以被AA还原，产生的开启发光可以反映AA的浓度。与纯Eu-pbmc晶体相比，PEM通过简洁的检测操作表现出改进的AA检测性能，检测限为48 nM，检测时间为1分钟。稳定的膜传感器实现了对实际生物样品的准确检测，满足了实际需求。此外，一个IMP逻辑门有助于分析水中的MnO和AA。所提出的新型发光平台以及合理的“开-关-开”发光模式为AA检测提供了一种有前景的方法。
