• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

熊果酸可保护卵母细胞免受衰老引起的退化，并改善猪的后续胚胎发育。

Asiatic acid protects oocytes against aging-induced deterioration and improves subsequent embryonic development in pigs.

机构信息

Department of Animals Sciences, College of Animal Sciences, Jilin University, Changchun, China.

College of Animal Science and Technology, Jilin Agriculture Science and Technology University, Changchun, China.

出版信息

Aging (Albany NY). 2020 Dec 3;13(3):3353-3367. doi: 10.18632/aging.202184.

DOI:10.18632/aging.202184
PMID:33281118
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7906213/
Abstract

As a pentacyclic triterpene in , asiatic acid (AA) is a powerful antioxidant with many bioactivities. In the present research, we investigated whether AA has the potential to rescue the decrease in porcine oocyte quality that occurs during aging (IVA). Mature porcine oocytes were collected and then continuously cultured for an additional 24 h or 48 h with or without AA in maturation medium as an IVA model. The results revealed that AA supplementation reduced the percentage of abnormal aged porcine oocytes during IVA. Furthermore, AA supplementation effectively maintained aged porcine oocyte developmental competence, both parthenogenetic activation and fertilization. The number of sperm that bound to the zona pellucida on aged porcine oocytes was higher in the AA-supplemented group than in the non-supplemented group. Moreover, AA supplementation not only blocked IVA-induced oxidative stress but also maintained intracellular GSH levels and reduced the percentage of early apoptosis aged porcine oocytes. Mitochondrial functions were disordered during the IVA process. The intracellular ATP levels and mitochondrial membrane potential in aged porcine oocytes were dramatically increased by AA supplementation. Therefore, AA has beneficial effects on porcine oocyte quality and developmental potential maintenance during IVA.

摘要

作为一种五环三萜，齐墩果酸（AA）是一种具有多种生物活性的强大抗氧化剂。在本研究中，我们研究了 AA 是否有潜力挽救猪卵母细胞在体外老化（IVA）过程中质量下降的问题。收集成熟的猪卵母细胞，然后在成熟培养基中继续培养额外的 24 小时或 48 小时，有或没有 AA，作为 IVA 模型。结果表明，AA 补充剂降低了 IVA 过程中异常老化猪卵母细胞的百分比。此外，AA 补充剂有效维持了老化猪卵母细胞的发育能力，包括孤雌激活和受精。在 AA 补充组中，与非补充组相比，结合到老化猪卵母细胞透明带的精子数量更高。此外，AA 补充不仅阻断了 IVA 诱导的氧化应激，还维持了细胞内 GSH 水平，并降低了早期凋亡老化猪卵母细胞的百分比。线粒体功能在 IVA 过程中失调。AA 补充剂显著增加了老化猪卵母细胞的细胞内 ATP 水平和线粒体膜电位。因此，AA 对 IVA 期间猪卵母细胞质量和发育潜能的维持具有有益作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/38abb0ef900b/aging-13-202184-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/d47ee4a6a760/aging-13-202184-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/acb7444dd0a7/aging-13-202184-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/ca67267ff41c/aging-13-202184-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/12a02f5821e2/aging-13-202184-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/0dca69209658/aging-13-202184-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/5d7b23513dca/aging-13-202184-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/d96baf85c1bd/aging-13-202184-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/8f1b85275499/aging-13-202184-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/542ba17b2540/aging-13-202184-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/38abb0ef900b/aging-13-202184-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/d47ee4a6a760/aging-13-202184-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/acb7444dd0a7/aging-13-202184-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/ca67267ff41c/aging-13-202184-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/12a02f5821e2/aging-13-202184-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/0dca69209658/aging-13-202184-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/5d7b23513dca/aging-13-202184-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/d96baf85c1bd/aging-13-202184-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/8f1b85275499/aging-13-202184-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/542ba17b2540/aging-13-202184-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3449/7906213/38abb0ef900b/aging-13-202184-g010.jpg

相似文献

1
Asiatic acid protects oocytes against aging-induced deterioration and improves subsequent embryonic development in pigs.熊果酸可保护卵母细胞免受衰老引起的退化，并改善猪的后续胚胎发育。
Aging (Albany NY). 2020 Dec 3;13(3):3353-3367. doi: 10.18632/aging.202184.
2
Supplementation with asiatic acid during in vitro maturation improves porcine oocyte developmental competence by regulating oxidative stress.在体外成熟过程中补充积雪草酸可通过调节氧化应激来提高猪卵母细胞的发育能力。
Theriogenology. 2021 Sep 15;172:169-177. doi: 10.1016/j.theriogenology.2021.06.013. Epub 2021 Jun 17.
3
Asiatic acid supplementation during the in vitro culture period improves early embryonic development of porcine embryos produced by parthenogenetic activation, somatic cell nuclear transfer and in vitro fertilization.在体外培养期间补充土槿乙酸可改善孤雌激活、体细胞核移植和体外受精产生的猪胚胎的早期胚胎发育。
Theriogenology. 2020 Jan 15;142:26-33. doi: 10.1016/j.theriogenology.2019.09.027. Epub 2019 Sep 19.
4
Mogroside V improves porcine oocyte in vitro maturation and subsequent embryonic development.蜜瓜苷 V 可提高猪卵母细胞体外成熟和随后的胚胎发育。
Theriogenology. 2020 Jan 1;141:35-40. doi: 10.1016/j.theriogenology.2019.09.010. Epub 2019 Sep 6.
5
Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs.泛醇-10 通过改善猪卵母细胞的线粒体更新来延缓排卵后卵母细胞衰老。
Aging (Albany NY). 2020 Jan 20;12(2):1256-1271. doi: 10.18632/aging.102681.
6
Melatonin prevents postovulatory oocyte aging and promotes subsequent embryonic development in the pig.褪黑素可防止猪排卵后卵母细胞老化，并促进随后的胚胎发育。
Aging (Albany NY). 2017 Jun 26;9(6):1552-1564. doi: 10.18632/aging.101252.
7
Mitochondria-targeted therapy rescues development and quality of embryos derived from oocytes matured under oxidative stress conditions: a bovine in vitro model.线粒体靶向治疗可挽救氧化应激条件下成熟卵母细胞来源胚胎的发育和质量：牛体外模型。
Hum Reprod. 2019 Oct 2;34(10):1984-1998. doi: 10.1093/humrep/dez161.
8
The effects of resveratrol on porcine oocyte in vitro maturation and subsequent embryonic development after parthenogenetic activation and in vitro fertilization.白藜芦醇对猪卵母细胞体外成熟以及孤雌激活和体外受精后胚胎发育的影响。
Theriogenology. 2012 Jul 1;78(1):86-101. doi: 10.1016/j.theriogenology.2012.01.024. Epub 2012 Mar 23.
9
Inhibition of cathepsin B activity prevents deterioration in the quality of in vitro aged porcine oocytes.抑制组织蛋白酶B的活性可防止体外老化猪卵母细胞质量下降。
Theriogenology. 2018 Aug;116:103-111. doi: 10.1016/j.theriogenology.2018.04.035. Epub 2018 May 4.
10
A pre-in vitro maturation medium containing cumulus oocyte complex ligand-receptor signaling molecules maintains meiotic arrest, supports the cumulus oocyte complex and improves oocyte developmental competence.含有卵丘卵母细胞复合物配体-受体信号分子的预体外成熟培养基可维持减数分裂阻滞，支持卵丘卵母细胞复合物，并提高卵母细胞发育能力。
Mol Hum Reprod. 2017 Sep 1;23(9):594-606. doi: 10.1093/molehr/gax032.

引用本文的文献

1
Ferulic acid ameliorates the quality of -aged bovine oocytes by suppressing oxidative stress and apoptosis.阿魏酸通过抑制氧化应激和凋亡改善衰老牛卵母细胞的质量。
Aging (Albany NY). 2023 Nov 8;15(21):12497-12512. doi: 10.18632/aging.205193.
2
Iron Overload-Induced Ferroptosis Impairs Porcine Oocyte Maturation and Subsequent Embryonic Developmental Competence .铁过载诱导的铁死亡损害猪卵母细胞成熟及随后的胚胎发育能力。
Front Cell Dev Biol. 2021 May 28;9:673291. doi: 10.3389/fcell.2021.673291. eCollection 2021.
3
Nrf2 Signaling Pathway in Chemoprotection and Doxorubicin Resistance: Potential Application in Drug Discovery.

本文引用的文献

1
Mogroside V protects porcine oocytes from ageing by reducing oxidative stress through SIRT1 upregulation.罗汉果甜苷V通过上调SIRT1减少氧化应激，从而保护猪卵母细胞免受老化。
Aging (Albany NY). 2019 Oct 6;11(19):8362-8373. doi: 10.18632/aging.102324.
2
Asiatic acid supplementation during the in vitro culture period improves early embryonic development of porcine embryos produced by parthenogenetic activation, somatic cell nuclear transfer and in vitro fertilization.在体外培养期间补充土槿乙酸可改善孤雌激活、体细胞核移植和体外受精产生的猪胚胎的早期胚胎发育。
Theriogenology. 2020 Jan 15;142:26-33. doi: 10.1016/j.theriogenology.2019.09.027. Epub 2019 Sep 19.
3
Nrf2信号通路在化学保护和多柔比星耐药中的作用：在药物研发中的潜在应用
Antioxidants (Basel). 2021 Feb 26;10(3):349. doi: 10.3390/antiox10030349.
Assisted Reproductive Technology Surveillance - United States, 2016.
辅助生殖技术监测报告——美国，2016 年。
MMWR Surveill Summ. 2019 Apr 26;68(4):1-23. doi: 10.15585/mmwr.ss6804a1.
4
Asiatic Acid Protects Dopaminergic Neurons from Neuroinflammation by Suppressing Mitochondrial Ros Production.齐墩果酸通过抑制线粒体活性氧生成保护多巴胺能神经元免受神经炎症影响。
Biomol Ther (Seoul). 2019 Sep 1;27(5):442-449. doi: 10.4062/biomolther.2018.188.
5
Laminarin enhances the quality of aged pig oocytes by reducing oxidative stress.海带多糖通过降低氧化应激来提高老龄猪卵母细胞的质量。
J Reprod Dev. 2018 Dec 14;64(6):489-494. doi: 10.1262/jrd.2018-031. Epub 2018 Oct 1.
6
Melatonin supplementation during prolonged in vitro maturation improves the quality and development of poor-quality porcine oocytes via anti-oxidative and anti-apoptotic effects.褪黑素补充剂在延长的体外成熟过程中通过抗氧化和抗凋亡作用提高了劣质猪卵母细胞的质量和发育能力。
Mol Reprod Dev. 2018 Aug;85(8-9):665-681. doi: 10.1002/mrd.23052. Epub 2018 Oct 5.
7
Asiatic Acid Prevents Oxidative Stress and Apoptosis by Inhibiting the Translocation of α-Synuclein Into Mitochondria.齐墩果酸通过抑制α-突触核蛋白转位至线粒体来预防氧化应激和细胞凋亡。
Front Neurosci. 2018 Jun 28;12:431. doi: 10.3389/fnins.2018.00431. eCollection 2018.
8
Glutathione deficiency sensitizes cultured embryonic mouse ovaries to benzo[a]pyrene-induced germ cell apoptosis.谷胱甘肽缺乏使培养的胚胎期小鼠卵巢对苯并[a]芘诱导的生殖细胞凋亡敏感。
Toxicol Appl Pharmacol. 2018 Aug 1;352:38-45. doi: 10.1016/j.taap.2018.05.024. Epub 2018 May 22.
9
Importance of ERK1/2 in Regulation of Protein Translation during Oocyte Meiosis.ERK1/2 在卵母细胞减数分裂过程中对蛋白质翻译的调节作用。
Int J Mol Sci. 2018 Mar 1;19(3):698. doi: 10.3390/ijms19030698.
10
Symposium review: Reduction in oocyte developmental competence by stress is associated with alterations in mitochondrial function.研讨会综述：应激导致卵母细胞发育能力下降与线粒体功能改变有关。
J Dairy Sci. 2018 Apr;101(4):3642-3654. doi: 10.3168/jds.2017-13389. Epub 2018 Feb 1.