Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.
Department of Advanced Pharmaceutics, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Hokkaido 060-0818, Japan.
J Vet Med Sci. 2021 Feb 25;83(2):162-166. doi: 10.1292/jvms.20-0590. Epub 2020 Dec 7.
Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we performed anti-PD-L1 antibody administration using 2 MAP-infected cattle at the late subclinical stage of infection. After administration, bacterial shedding was reduced or maintained at a low level. Additionally, MAP-specific Th1 cytokine production was upregulated, and CD69 expression was increased in T cells. Collectively, the treatment has a potential as a novel control method against Johne's disease.
副结核病由鸟分枝杆菌副结核亚种（MAP）引起，是反刍动物的一种慢性肠炎。先前的研究表明，程序性死亡配体 1（PD-L1）与疾病进展有关，PD-L1 阻断在体外激活 MAP 特异性 Th1 反应。在这里，我们在感染后期的亚临床阶段对 2 头感染 MAP 的牛进行了抗 PD-L1 抗体给药。给药后，细菌脱落减少或维持在低水平。此外，MAP 特异性 Th1 细胞因子的产生上调，T 细胞中 CD69 的表达增加。总的来说，该治疗方法具有作为一种针对副结核病的新型控制方法的潜力。
