Suprayogi Tri Wahyu, Hardijanto Hardijanto, Hariadi Mas'ud, Rantam Fedik Abdul, Darmanto Win
Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Department of Microbiology and Virology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya 60115, Indonesia.
Vet World. 2020 Oct;13(10):2112-2117. doi: 10.14202/vetworld.2020.2112-2117. Epub 2020 Oct 9.
The implementation of artificial insemination (AI) is one of the strategies to use superior male semen optimally to improve the genetic quality of livestock. One of the factors that influence AI is a fertility-associated antigen (FAA). This research aimed to examine the effects of FAA extracted from the accessory sex glands of a bull from a slaughterhouse that was added in bull semen freezing medium to increase cattle (bull) fertilization.
This research used a randomized complete block design. It consisted of two research phases, namely, explorative and experimental phases. The first phase involved determining the FAA molecular weight using the SDS-PAGE method, and the second phase consisted of laboratory and field testing, including testing the quality of frozen semen supplemented with FAA extracted from the accessory glands of a bull's genital organ from a slaughterhouse with various doses (0, 5, 10, and 15 μg in every 200 million progressively motile spermatozoa).
The results showed that the percentages of bull sperm motility between the groups without and with the additional administration of FAA with a dose of 5 μg did not significantly differ. However, there was a difference between the groups without and with the additional administration of FAA with doses of 10 and 15 μg. After further testing, the highest percentage of sperm progressive motility occurred at a dose of 15 μg/200 million progressively motile spermatozoa (P3), which was equal to 2.59±46.88b (%).
This research found that not all of the accessory glands (seminal vesicles) of bulls taken from the slaughterhouse contain the FAA. An FAA level between the accessory glands (seminal vesicles) of one cattle to another is different. The addition of the FAA protein from the accessory sex glands of a bull's organ in cattle semen can improve fertility by increasing the percentage of viability, motility, intact plasma membrane of spermatozoa, and pregnancy rate of bulls and decreasing the sperm capacitation post-thawing.
实施人工授精（AI）是优化使用优良公畜精液以提高家畜遗传质量的策略之一。影响人工授精的因素之一是生育相关抗原（FAA）。本研究旨在检测从屠宰场公牛副性腺中提取的FAA添加到公牛精液冷冻培养基中对提高牛（公牛）受精率的影响。
本研究采用随机完全区组设计。它包括两个研究阶段，即探索阶段和实验阶段。第一阶段使用SDS-PAGE方法确定FAA分子量，第二阶段包括实验室和现场测试，包括检测添加从屠宰场公牛生殖器官副性腺中提取的不同剂量（每2亿条进行性运动精子中分别添加0、5、10和15μg）FAA的冷冻精液质量。
结果表明，未添加FAA组与添加5μg剂量FAA组之间公牛精子活力百分比无显著差异。然而，未添加FAA组与添加10μg和15μg剂量FAA组之间存在差异。进一步检测后，精子进行性运动的最高百分比出现在15μg/2亿条进行性运动精子的剂量组（P3），为2.59±46.88b（%）。
本研究发现，并非所有从屠宰场获取的公牛副性腺（精囊）都含有FAA。一头牛与另一头牛的副性腺（精囊）之间的FAA水平不同。在牛精液中添加公牛器官副性腺中的FAA蛋白可通过提高精子活力、运动能力、完整质膜百分比和公牛妊娠率以及降低解冻后精子获能来提高受精率。
