Gibbs N K
Department of Photobiology, Institute of Dermatology, UMDS, London, UK.
Photodermatol. 1987 Dec;4(6):312-6.
The Candida albicans assay is widely used for the testing of potential photosensitizers, especially furocoumarins. A novel adaptation of this assay is described which provides a rapid, inexpensive and easy-to-perform method of producing action spectra. The use of filter paper rectangles with central apertures instead of the usual discs, and monochromatic irradiation with a liquid light guide reduces some of the problems associated with the standard assay. Action spectra for 8-methoxypsoralen and a new pyrrolocoumarin derivative are presented as illustrative examples.
白色念珠菌检测法被广泛用于潜在光敏剂的测试，尤其是呋喃香豆素类。本文描述了该检测法的一种新应用，它提供了一种快速、廉价且易于操作的产生作用光谱的方法。使用带有中心孔的矩形滤纸而非通常的圆盘，并通过液体光导进行单色照射，减少了与标准检测法相关的一些问题。作为示例给出了8-甲氧基补骨脂素和一种新的吡咯并香豆素衍生物的作用光谱。
