Feng Zhongxin, Wang Jishi
Department of Clinical Medical School, Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China.
Department of Hematology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China.
Oncol Lett. 2021 Jan;21(1):56. doi: 10.3892/ol.2020.12318. Epub 2020 Nov 18.
The incidence of non-Hodgkin's lymphoma (NHL) has been increasing annually and has become a serious threat to human health. However, the pathogenesis of NHL remains unclear. The present study aimed to investigate the effect of soluble CD40 ligand (sCD40L) on NHL cells and its underlying mechanism. Cell Counting kit-8 assay and flow cytometry apoptosis experiments were conducted to investigate the effects of sCD40L on cell proliferation and apoptosis. Western blotting was performed to detect the protein expression levels of BAX, Bcl-2, ERK, p-ERK, JNK, p-JNK, p38, p-p38 and c-JUN. The results of the present study demonstrated that exogenous sCD40L significantly inhibited the proliferation and promoted the apoptosis of Raji and CA46 cells. Additionally, exogenous sCD40L promoted the apoptosis of lymphoma cells by activating the JNK signaling pathway.
非霍奇金淋巴瘤（NHL）的发病率逐年上升，已对人类健康构成严重威胁。然而，NHL的发病机制仍不清楚。本研究旨在探讨可溶性CD40配体（sCD40L）对NHL细胞的影响及其潜在机制。采用细胞计数试剂盒-8法和流式细胞术凋亡实验研究sCD40L对细胞增殖和凋亡的影响。通过蛋白质印迹法检测BAX、Bcl-2、ERK、p-ERK、JNK、p-JNK、p38、p-p38和c-JUN的蛋白表达水平。本研究结果表明，外源性sCD40L显著抑制Raji和CA46细胞的增殖并促进其凋亡。此外，外源性sCD40L通过激活JNK信号通路促进淋巴瘤细胞凋亡。
