Feng Zhong-Xin, Wang Ji-Shi, Chen Qi
Department of Hematology of Affiliated Hospital of Zunyi Medical University, Zunyi 563003, Guizhou Province, China,Guizhou Medical University Graduate School, Guiyang 550004, Guizhou Province, China.
Department of Hematology of Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Dec;28(6):1933-1938. doi: 10.19746/j.cnki.issn.1009-2137.2020.06.023.
To explore the effect of soluble CD40 ligand (sCD40L) on the proliferation, apoptosis, and cell cycle of human non-Hodgkin lymphoma (NHL) cells, and analyze its possible mechanism.
NHL CA46 cell and Raji cell were treated with different concentrations of sCD40L for 48 h, CCK-8 was used to detect the effect of sCD40L on cell proliferation in vitro, flow cytometry on apoptosis and cycle of NHL cells, and Western blot on the expression of PTEN, BCL-2, and BAX in NHL cells.
Compared with the control group, 4 and 8 μg/ml sCD40L could significantly inhibit the proliferation of lymphoma Raji cell and CA46 cell (P<0.05). The test results of flow cytometry showed that 4 μg/ml sCD40L could significantly promote the apoptosis of CA46 and Raji cells, and significantly inhibit the S phase proportions (P<0.05). Western blot results showed that sCD40L could promote the expression of PTEN and BAX, while inhibit the expression of BCL-2 (P<0.05).
sCD40L can promote the apoptosis and inhibit the proliferation of NHL cells through the PTEN signaling pathway.
探讨可溶性CD40配体（sCD40L）对人非霍奇金淋巴瘤（NHL）细胞增殖、凋亡及细胞周期的影响，并分析其可能机制。
用不同浓度的sCD40L处理NHL CA46细胞和Raji细胞48小时，采用CCK-8检测sCD40L对细胞体外增殖的影响，流式细胞术检测NHL细胞凋亡和周期，蛋白质免疫印迹法检测NHL细胞中PTEN、BCL-2和BAX的表达。
与对照组相比，4和8μg/ml的sCD40L可显著抑制淋巴瘤Raji细胞和CA46细胞的增殖（P<0.05）。流式细胞术检测结果显示，4μg/ml的sCD40L可显著促进CA46和Raji细胞凋亡，并显著抑制S期比例（P<0.05）。蛋白质免疫印迹结果显示，sCD40L可促进PTEN和BAX的表达，同时抑制BCL-2的表达（P<0.05）。
sCD40L可通过PTEN信号通路促进NHL细胞凋亡并抑制其增殖。
