Yamasaki Masao, Minesaki Mikako, Iwakiri Asuka, Miyamoto Yuko, Ogawa Kenjiro, Nishiyama Kazuo, Tsend-Ayush Chuluunbat, Oyunsuren Tsendesuren, Li Yiran, Nakano Tomoki, Takeshita Masahiko, Arima Yuo
Graduate School of Agriculture University of Miyazaki Miyazaki Japan.
Organization for Promotion of Tenure Track University of Miyazaki Miyazaki Japan.
Food Sci Nutr. 2020 Oct 26;8(11):6164-6173. doi: 10.1002/fsn3.1909. eCollection 2020 Nov.
Previous study suggested that dietary intake of 06CC2 (LP06CC2) isolated from Mongolian dairy products showed various health beneficial effects. Here, the effect of LP06CC2 on the cholesterol metabolism in mice fed a cholesterol-loaded diet was evaluated. Cholesterol and LP06CC2 were incorporated into the AIN93G-based diet to evaluate the effect on cholesterol metabolism in Balb/c mice. Serum and liver cholesterol levels were significantly increased in mice fed a cholesterol-loaded diet whereas the LP06CC2 ingestion suppressed the increase of liver cholesterol. LP06CC2 suppressed the increase of the hepatic damage indices. The increase of the cecal content and fecal butyrate were observed in mice fed LP06CC2. The analysis of bile acids clearly showed that LP06CC2 increased their deconjugation indicating the decrease of bile acid absorption. The protein expression of hepatic Cyp7A1 was also suppressed by LP06CC2 in mice fed cholesterol. Finally, in vitro studies showed that LP06CC2 had the most potent ability to deconjugate bile acids using glycocholate among the tested probiotic lactic acid bacteria isolated from Mongolian dairy products. Taken together, LP06CC2 is a promising microorganism for the reduction of the cholesterol pool via modulation of bile acid deconjugation.
先前的研究表明，从蒙古乳制品中分离出的06CC2（LP06CC2）的膳食摄入具有多种有益健康的作用。在此，评估了LP06CC2对喂食高胆固醇饮食的小鼠胆固醇代谢的影响。将胆固醇和LP06CC2添加到基于AIN93G的饮食中，以评估对Balb/c小鼠胆固醇代谢的影响。喂食高胆固醇饮食的小鼠血清和肝脏胆固醇水平显著升高，而摄入LP06CC2可抑制肝脏胆固醇的升高。LP06CC2抑制了肝脏损伤指标的升高。在喂食LP06CC2的小鼠中观察到盲肠内容物和粪便丁酸盐增加。胆汁酸分析清楚地表明，LP06CC2增加了它们的去结合作用，表明胆汁酸吸收减少。LP06CC2还抑制了喂食胆固醇的小鼠肝脏Cyp7A1的蛋白表达。最后，体外研究表明，在从蒙古乳制品中分离出的测试益生菌乳酸菌中，LP06CC2具有使用甘氨胆酸盐去结合胆汁酸的最强能力。综上所述，LP06CC2是一种有前景的微生物，可通过调节胆汁酸去结合作用来减少胆固醇池。
