University of Teramo, Faculty of Bioscience and Technology for Food, Agriculture and Environment, 64100, Via Balzarini 1, Teramo, Italy.
APC Microbiome Ireland, University College Cork, Cork, Ireland.
Sci Rep. 2020 Jan 24;10(1):1165. doi: 10.1038/s41598-020-58069-5.
Bile acid (BA) signatures are altered in many disease states. BA metabolism is an important microbial function to assist gut colonization and persistence, as well as microbial survival during gastro intestinal (GI) transit and it is an important criteria for potential probiotic bacteria. Microbes that express bile salt hydrolase (BSH), gateway BA modifying enzymes, are considered to have an advantage in the gut. This property is reported as selectively limited to gut-associated microbes. Food-associated microbes have the potential to confer health benefits to the human consumer. Here, we report that food associated Lactobacillus plantarum strains are capable of BA metabolism, they can withstand BA associated stress and propagate, a recognised important characteristic for GIT survival. Furthermore, we report that these food associated Lactobacillus plantarum strains have the selective ability to alter BA signatures in favour of receptor activation that would be beneficial to humans. Indeed, all of the strains examined showed a clear preference to alter human glycol-conjugated BAs, although clear strain-dependent modifications were also evident. This study demonstrates that BA metabolism by food-borne non-pathogenic bacteria is beneficial to both microbe and man and it identifies an evolutionary-conserved characteristic, previously considered unique to gut residents, among food-associated non-pathogenic isolates.
胆汁酸 (BA) 特征在许多疾病状态下发生改变。BA 代谢是一种重要的微生物功能，可以帮助肠道定植和持续存在，以及微生物在胃肠道 (GI) 转运过程中的存活，这是潜在益生菌细菌的一个重要标准。表达胆汁盐水解酶 (BSH) 的微生物，即 BA 修饰酶的门户，被认为在肠道中具有优势。据报道，这种特性仅限于与肠道相关的微生物。与食物相关的微生物有可能为人类消费者带来健康益处。在这里，我们报告称，与食物相关的植物乳杆菌菌株能够进行 BA 代谢，它们能够承受与 BA 相关的压力并繁殖，这是 GIT 存活的一个公认的重要特征。此外，我们报告称，这些与食物相关的植物乳杆菌菌株具有选择性地改变 BA 特征的能力，有利于受体激活，这对人类有益。事实上，所有被检查的菌株都显示出明显的偏好，即改变人类糖结合的 BA，尽管也明显存在菌株依赖性的修饰。这项研究表明，食源性病原体非致病性细菌的 BA 代谢对微生物和人类都有益，它确定了一种进化保守的特征，以前被认为是肠道居民所特有的，而在与食物相关的非致病性分离物中也存在这种特征。
