Hassanien Mohammed Ahmed
Department of Pharmacy Practice, College of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Medical Biochemistry, Faculty of Medicine, Tanta University, Tanta, Egypt.
J Microsc Ultrastruct. 2020 May 8;8(3):96-103. doi: 10.4103/JMAU.JMAU_70_19. eCollection 2020 Jul-Sep.
Myocardial infarction is a major heart disease and is considered a significant reason for mortality and morbidity around the world. The model of Isoproterenol (ISO)-induced myocardial infarction provides a supported method for investigating the impacts of numerous possible cardioprotective bioactive substances. Nitric Oxide (NO) could react with reactive oxygen intermediates and free radicals to create harmful species. For several years, researchers have investigated the use of herbs and natural products as antioxidants to protect the body's organs against toxins and drug metabolites. However, studies on the antioxidant effects of ginger against cardiotoxicity induced by drugs and toxic agents remain insufficient, especially its effects on NO.
This study aimed to investigate the possible antioxidant and protective role of ginger in ISO-induced acute myocardial infarction in experimental rats. Special emphasis was given to the impact of ginger on NO levels.
Forty adult male albino rats were used in this study. The animals were randomly divided into four equal groups. Group I served as control and received a normal mouse diet. Group II received ginger extract orally, Group III received normal diet for eight weeks, followed by ISO administration subcutaneously to induce myocardial infarction, Group IV received ginger extracts, followed by ISO.
The results of this study illustrated ginger's protective role against ISO-induced acute myocardial infarction. This role is mainly due to ginger's antioxidant and anti-inflammatory properties. We assume that sufficient intake of ginger by individuals who are regularly exposed to ISO would be beneficial in overcoming the cardiotoxicity of ISO. The effects of ginger may take place through inhibition of NOS enzymes, which needs further immunohistochemical and biochemical studies to reveal the underlying different mechanisms of the effects of ginger at the molecular and structural levels.
心肌梗死是一种主要的心脏病，被认为是全球死亡率和发病率的重要原因。异丙肾上腺素（ISO）诱导的心肌梗死模型为研究多种可能的心脏保护生物活性物质的影响提供了一种可靠的方法。一氧化氮（NO）可与活性氧中间体和自由基反应生成有害物质。多年来，研究人员一直在研究使用草药和天然产物作为抗氧化剂来保护身体器官免受毒素和药物代谢产物的侵害。然而，关于生姜对药物和有毒物质诱导的心脏毒性的抗氧化作用的研究仍然不足，尤其是其对NO的影响。
本研究旨在探讨生姜在实验大鼠ISO诱导的急性心肌梗死中可能的抗氧化和保护作用。特别强调生姜对NO水平的影响。
本研究使用了40只成年雄性白化大鼠。动物被随机分为四组，每组数量相等。第一组作为对照组，给予正常小鼠饮食。第二组口服生姜提取物，第三组先给予正常饮食8周，然后皮下注射ISO诱导心肌梗死，第四组先给予生姜提取物，然后注射ISO。
本研究结果表明生姜对ISO诱导的急性心肌梗死具有保护作用。这种作用主要归因于生姜的抗氧化和抗炎特性。我们认为，经常接触ISO的个体充分摄入生姜将有助于克服ISO的心脏毒性。生姜的作用可能是通过抑制NOS酶来实现的，这需要进一步的免疫组织化学和生化研究来揭示生姜在分子和结构水平上作用的潜在不同机制。
