Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Annamalainagar, Tamil Nadu, India.
Drug Dev Res. 2019 Sep;80(6):814-823. doi: 10.1002/ddr.21564. Epub 2019 Jul 16.
The present study aimed at investigating the protective effects of nerolidol (NRD) against myocardial infarction (MI) induced by isoproterenol (ISO) in Wistar rats. The rats were randomly divided into five groups, each group consisting of six rats. Group I were treated as control rats, group II received NRD (200 mg/kg b.w.) by intragastric intubation for 21 days, group III received ISO (60 mg/kg b.w) subcutaneously (s.c) for two consecutive days on 22nd and 23rd day, group IV and V received NRD (100 and 200 mg/kg b.w) as in group II and additionally ISO was given for two consecutive days (22nd and 23rd). On 24th day all the rats were sacrificed by cervical dislocation and the blood and heart samples were collected. In the present study, ISO-induced myocardial damage was indicated by the changes in body weight, heart weight and the cardiac and hepatic marker enzymes such as creatine kinase (CK), creatine kinase-MB (CK-MB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and troponin T and I (cTnT, cTnI) in the serum. In addition, the levels of lipid peroxidation products such as thiobarbituric acid reactive substances (TBARS), conjugated dines (CD), and lipid hydroperoxides (LHPs) increased significantly in the plasma and heart tissue. Activities of enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) in erythrocytes and heart tissue and the levels of nonenzymatic antioxidants like vitamin C, vitamin E, and reduced glutathione (GSH) in plasma and heart tissue were decreased in ISO-induced rats. Histopathological observations were also supported with the biochemical parameters. Pretreatment with NRD at different doses (100 and 200 mg/kg b.w) for 21 days prevented the above changes induced by ISO. The 200 mg/kg b.w of NRD was more pronounced than the other dose and brought back all the above parameters near to normalcy.
本研究旨在探讨橙花叔醇（NRD）对异丙肾上腺素（ISO）诱导的 Wistar 大鼠心肌梗死（MI）的保护作用。将大鼠随机分为五组，每组 6 只。第 I 组作为对照，第 II 组连续 21 天每天经胃管给予 NRD（200mg/kg bw），第 III 组连续 2 天（第 22 天和第 23 天）每天皮下给予 ISO（60mg/kg bw），第 IV 组和第 V 组分别以第 II 组相同的方式给予 NRD（100 和 200mg/kg bw），并额外给予 ISO 连续 2 天（第 22 天和第 23 天）。第 24 天，所有大鼠通过颈椎脱位处死，收集血液和心脏样本。在本研究中，ISO 诱导的心肌损伤表现为体重、心脏重量以及血清中心脏和肝脏标志物酶如肌酸激酶（CK）、肌酸激酶同工酶-MB（CK-MB）、丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、乳酸脱氢酶（LDH）和肌钙蛋白 T 和 I（cTnT、cTnI）的变化。此外，血浆和心脏组织中脂质过氧化产物如硫代巴比妥酸反应物质（TBARS）、共轭二烯（CD）和脂质过氧化物（LHPs）的水平显著升高。红细胞和心脏组织中抗氧化酶如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）、谷胱甘肽-S-转移酶（GST）的活性以及血浆和心脏组织中非酶抗氧化剂如维生素 C、维生素 E 和还原型谷胱甘肽（GSH）的水平在 ISO 诱导的大鼠中降低。组织病理学观察也支持生化参数。用 NRD（100 和 200mg/kg bw）预处理 21 天可预防 ISO 引起的上述变化。200mg/kg bw 的 NRD 比其他剂量更显著，使上述所有参数恢复接近正常。
