Finol Héctor J, Garcia-Lunardi Estefanie, González Roschman, Girón Maria E, Uzcátegui Nestor L, Rodríguez-Acosta Alexis
Microscopy Electronic Centre, Faculty of Sciences, Universidad Central De Venezuela, Caracas, Venezuela.
Anatomical Institute, Immunochemistry and Ultrastructure Laboratory, Universidad Central De Venezuela, Caracas, Venezuela.
J Microsc Ultrastruct. 2020 Sep 10;8(3):104-114. doi: 10.4103/JMAU.JMAU_49_19. eCollection 2020 Jul-Sep.
The damage of the adrenal gland by snake venoms needs to be clarified. Lethality (LD) of () venom was established by intraperitoneally mice injections. Preparation of specimens for transmission electron microscopy samples from cortex adrenal gland biopsies at 3, 6, and 24 h was processed. The quantitative description by the principal component analysis (PCA) of the adrenal gland was as follows: thickening of the capillary endothelium, area of the capillary lumen, cell nucleus area, enlargement of the perinuclear space, number of mitochondria, area of the mitochondria, number of mitochondrial cristae, number of cristae per mitochondrial unit, and tubular diameter of the smooth endoplasmic reticulum (SER). Sections of the adrenal cortex, after 3 h postinjection with venom showed in the cortical cells: mitochondria with tubular cristae and slightly swollen SER cisternae, nucleus with variable heterochromatin content, irregular edges, and swollen nuclear envelope. After 6 h, cells with swollen nucleus envelope, electron dense lipids and mitochondria with loss of their cristae were observed. Myelin figures, close to the microvilli of the cortical cell, multivesicular bodies, swollen profiles of the SER, and electron dense lipid drops were noticed. After 24 h, thickening of the endothelial wall, fenestrae and projections into the capillary lumen, loss of the mitochondrial cristae, destruction of the capillary and the plasma membrane of the cortical cell, multivesicular body, SER loss, and an enlargement of the perinuclear space were detected. In the quantitative PCA, there were significant changes after the venom treatments.
蛇毒对肾上腺的损害需要阐明。通过腹腔注射小鼠确定了（）毒液的致死率（LD）。对肾上腺皮质活检标本在3小时、6小时和24小时进行透射电子显微镜样本制备。通过主成分分析（PCA）对肾上腺进行的定量描述如下：毛细血管内皮增厚、毛细血管腔面积、细胞核面积、核周间隙增大、线粒体数量、线粒体面积、线粒体嵴数量、每个线粒体单位的嵴数量以及滑面内质网（SER）的管径。注射毒液3小时后，肾上腺皮质切片显示皮质细胞中的线粒体具有管状嵴且SER池轻度肿胀，细胞核的异染色质含量可变，边缘不规则，核膜肿胀。6小时后，观察到细胞核膜肿胀、电子致密脂质以及线粒体嵴消失的细胞。靠近皮质细胞微绒毛的髓鞘样结构、多泡体、SER肿胀轮廓以及电子致密脂滴被注意到。24小时后，检测到内皮细胞壁增厚、窗孔和向毛细血管腔内的突起、线粒体嵴消失、毛细血管和皮质细胞质膜破坏、多泡体、SER丧失以及核周间隙增大。在定量PCA中，毒液处理后有显著变化。
