Escola de Veterinária, Departamento de Clínica e Cirurgia Veterinária, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, Brazil.
Faculdade de Medicina Veterinária e Zootecnia, Departamento de Medicina Veterinária Preventiva e Saúde Animal (VPS), Universidade de São Paulo (USP), São Paulo, SP, Brazil.
J Med Primatol. 2021 Apr;50(2):99-107. doi: 10.1111/jmp.12506. Epub 2020 Dec 6.
Toxoplasmosis is a zoonotic disease that affects humans and warm-blooded animals. This study describes an outbreak of toxoplasmosis in howler monkeys (Alouatta sp.) and survival of capuchins (Sapajus apella), under the same environmental conditions.
Howler monkeys were submitted to post-mortem examination. Tissue samples were processed to histopathology and immunohistochemistry to detect lesions and tachyzoites of Toxoplasma gondii. Tissue samples were also frozen and submitted to PCR and genotyping of T. gondii.
Typical lesions were observed in several organs including the liver, lymph node, and brain, with intralesional cysts and tachyzoites of T. gondii demonstrated by immunohistochemistry. T. gondii genomic sequences were amplified by PCR, and genotyping characterized the same T. gondii clone in all howler monkeys.
Our results support the notion that some species of neotropical primates are highly susceptible to toxoplasmosis and the hypothesis that capuchins (S. apella) may be resistant.
弓形虫病是一种影响人类和温血动物的人畜共患病。本研究描述了在相同环境条件下，吼猴（Alouatta sp.）爆发弓形虫病和卷尾猴（Sapajus apella）存活的情况。
对吼猴进行剖检。组织样本进行组织病理学和免疫组织化学处理，以检测弓形虫（Toxoplasma gondii）的病变和速殖子。组织样本也被冷冻，并进行 T. gondii 的 PCR 和基因分型。
在包括肝脏、淋巴结和大脑在内的几个器官中观察到典型的病变，免疫组织化学显示有组织内囊肿和 T. gondii 的速殖子。通过 PCR 扩增了 T. gondii 的基因组序列，基因分型表明所有吼猴都具有相同的 T. gondii 克隆。
我们的结果支持一些新热带灵长类动物对弓形虫病高度易感的观点，以及卷尾猴（S. apella）可能具有抗性的假设。
