评价一种新开发的用于内脏利什曼病血清学诊断和血清流行病学研究的直接凝集试验（DAT）：与间接荧光抗体试验（IFAT）和酶联免疫吸附测定（ELISA）的比较。
Evaluation of a newly developed direct agglutination test (DAT) for serodiagnosis and sero-epidemiological studies of visceral leishmaniasis: comparison with IFAT and ELISA.
作者信息
Harith A E, Kolk A H, Kager P A, Leeuwenburg J, Faber F J, Muigai R, Kiugu S, Laarman J J
机构信息
Swellengrebel Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.
出版信息
Trans R Soc Trop Med Hyg. 1987;81(4):603-6. doi: 10.1016/0035-9203(87)90423-8.
A newly developed direct agglutination test (DAT) for visceral leishmaniasis, IFAT and ELISA were applied to sera of patients with visceral leishmaniasis, African and American trypanosomiasis, other parasitic infections and healthy controls. The sensitivities of the 3 tests were comparable (96.3% to 100%); excluding patients with African and American trypanosomiasis, the specificities of DAT and IFAT were 100% and ELISA 87.3%. When trypanosomiasis sera were included, the specificities were 72.6%, 94.3% and 79.4% in DAT, IFAT and ELISA respectively. In 273 sera from a leishmaniasis endemic area (Baringo District, Kenya), the sensitivity was 80% in DAT and IFAT and 60% in ELISA, specificities being 99.6% (DAT), 98.5% (IFAT) and 62.5% (ELISA). As the new DAT is economical and easy to perform, it is recommended for sero-epidemiological field work on visceral leishmaniasis.
一种新开发的用于内脏利什曼病的直接凝集试验（DAT）、间接荧光抗体试验（IFAT）和酶联免疫吸附测定（ELISA）被应用于内脏利什曼病患者、非洲锥虫病和美洲锥虫病患者、其他寄生虫感染患者以及健康对照者的血清检测。这三种检测方法的敏感性相当（96.3%至100%）；排除非洲锥虫病和美洲锥虫病患者后，DAT和IFAT的特异性为100%，ELISA的特异性为87.3%。当纳入锥虫病血清时，DAT、IFAT和ELISA的特异性分别为72.6%、94.3%和79.4%。在来自利什曼病流行地区（肯尼亚巴林戈区）的273份血清中，DAT和IFAT的敏感性为80%，ELISA的敏感性为60%，特异性分别为99.6%（DAT）、98.5%（IFAT）和62.5%（ELISA）。由于新的DAT经济且易于操作，推荐用于内脏利什曼病的血清流行病学现场工作。
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