Cheng Ming-Xia, Yu Shang-Rui, Wang Zhan-Dong, Bai Kun-Tian, Wang Dong-Ping, Li Juan, Liu Shi-Dong, Sun Yan-Qing, Dong Li
Department of Hematology, Gansu Provincial People's Hospital ;Department of Clinical Medicine, Gansu University of Chinese Medicine;Lanzhou 730000, Gansu Province, China.
Department of Digestion, The Second Hospital of Lanzhou University; Lanzhou 730000, Gansu Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2020 Dec;28(6):1885-1891. doi: 10.19746/j.cnki.issn.1009-2137.2020.06.016.
To investigated the anti-tumor in vivo effect and mechanism of the acid RNA protein complex (FA-2-b-β) of Agaricus blazei Murrill extract.
CCK-8 method was used to detected the inhibitory effect of FA-2-b-β on proliferation of primary CML cells from newly diagnosed CML patients, the CML mouse model was established by trail-venous injection of primary CML cells, and the survival time, blood cell count and body weight were observed, the immunoflouresence and immunehistochemistry analysis, RT-qPCR, Western bolt were used to detemine the expression of caspase-3 signal pathway-related apoptosis genes and proteins.
The experiments in vitro showed that the proliferative inhibitory rate in drug-treated group increased with concentration- and time-dependent manner (r=0.9092, r=0.9442, r=0.9546), the inter group comparison showed the statistical difference of results. The experiments in vitro showed that the survival time prolonged, blood cell count increased and body weight recovered in FA-2-b-β-treated group and imatinib-treated group, despite the WBC count is still high. The RT-qPCR and Western blot showed that the expression of BAX and caspase-3 gene and protein were up-regulated, the expression of BCL-2, cytochroime C, caspase-8, caspase-9 and BCL-ABL gene and protein were down-regulated.
The FA-2-b-β can induce apoptosis of primary CML cells and prolong the survival time of CML model mouse, which may be related with the caspase-3 signal pathway related genes and proteins.
研究姬松茸提取物酸性RNA蛋白复合物（FA-2-b-β）的体内抗肿瘤作用及机制。
采用CCK-8法检测FA-2-b-β对新诊断慢性粒细胞白血病（CML）患者原代CML细胞增殖的抑制作用，经尾静脉注射原代CML细胞建立CML小鼠模型，观察其生存时间、血细胞计数和体重，采用免疫荧光和免疫组化分析、RT-qPCR、Western blot检测caspase-3信号通路相关凋亡基因和蛋白的表达。
体外实验显示，药物处理组的增殖抑制率呈浓度和时间依赖性增加（r=0.9092，r=0.9442，r=0.9546），组间比较结果有统计学差异。体内实验显示，FA-2-b-β处理组和伊马替尼处理组的生存时间延长，血细胞计数增加，体重恢复，尽管白细胞计数仍较高。RT-qPCR和Western blot结果显示，BAX和caspase-3基因及蛋白表达上调，BCL-2、细胞色素C、caspase-8、caspase-9和BCL-ABL基因及蛋白表达下调。
FA-2-b-β可诱导原代CML细胞凋亡，延长CML模型小鼠的生存时间，其机制可能与caspase-3信号通路相关基因和蛋白有关。
