实时体外蓝藻生物钟的荧光各向异性。
Real-Time In Vitro Fluorescence Anisotropy of the Cyanobacterial Circadian Clock.
机构信息
Chemistry and Chemical Biology, University of California, Merced, CA, USA.
Center for Cellular and Biomolecular Machines, University of California, Merced, CA, USA.
出版信息
Methods Mol Biol. 2021;2130:3-18. doi: 10.1007/978-1-0716-0381-9_1.
Stochastic diffusion of a solution of fluorophores after photoselection reduces the polarization of emission, or fluorescence anisotropy. Because this randomization process is slower for larger molecules, fluorescence anisotropy is effective for measuring the kinetics of protein-binding events. Here, we describe how to use the technique to carry out real-time observations in vitro of the cyanobacterial circadian clock.
光选择后荧光团溶液的随机扩散会降低发射的偏振度，即荧光各向异性。由于这个随机化过程对于较大的分子较慢，因此荧光各向异性可有效地用于测量蛋白质结合事件的动力学。在这里，我们描述如何使用该技术在体外实时观察蓝细菌生物钟。
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