多孔多电极阵列记录下丘脑脑片。

Perforated Multi-Electrode Array Recording in Hypothalamic Brain Slices.

机构信息

Faculty of Biology, Medicine, and Health, AV Hill Building, University of Manchester, Manchester, UK.

Institute of Biomedical and Clinical Science, University of Exeter Medical School, Exeter, UK.

出版信息

Methods Mol Biol. 2021;2130:263-285. doi: 10.1007/978-1-0716-0381-9_20.

DOI:10.1007/978-1-0716-0381-9_20

PMID:33284451

Abstract

The ability to record ensemble action potential (AP) discharge frequencies from large populations of neurons over extended periods of time in vitro offers clear advantages in neuroscience and circadian biology research. Here, we provide an overview of a step-by-step method to perform multisite extracellular AP activity recordings in suprachiasmatic and mediobasal hypothalamic nuclei brain slices, using a state-of-the-art perforated multielectrode array system. Further, we describe in detail a setup architecture which systematically delivers stable, high-quality recordings with excellent anatomical accuracy and consistency. We also provide some procedural, technical, and methodological troubleshooting notes and examples of good quality recordings.

摘要

在体外长时间记录大群神经元的总和动作电位 (AP) 放电频率在神经科学和昼夜节律生物学研究中具有明显的优势。在这里，我们提供了一个分步的方法概述，用于使用最先进的穿孔多电极阵列系统在视交叉上核和中脑下丘脑核脑片中进行多部位细胞外 AP 活动记录。此外，我们详细描述了一种设置架构，该架构可系统地提供具有出色解剖准确性和一致性的稳定、高质量记录。我们还提供了一些程序、技术和方法学故障排除说明以及高质量记录的示例。