Department of Life Sciences, College of Natural Sciences, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.
Department of Bionanotechnology and Bioconvergence Engineering, Graduate School, Jeonbuk National University, 567 Baekje-daero, Deokjin-gu, Jeonju-si, Jeollabuk-do 54896, Republic of Korea.
Anal Chem. 2021 Jan 19;93(2):801-811. doi: 10.1021/acs.analchem.0c03196. Epub 2020 Dec 7.
An easily accessible colorimetric and fluorescence probe 4-((3-chloro-1,4-dioxo-1,4-dihydronaphthalen-2-yl)amino)benzenesulfonamide () was successfully developed for the selective and sensitive detection of Sn in an aqueous solution. The sensing mechanism involves reduction of -C═O into -C-OH groups in upon the addition of Sn, which initiates the fluorescence turn-on mode. A better linear relationship was achieved between fluorescence intensity and Sn concentration in the range of 0-62.5 μM, with a detection limit (LOD) of 0.115 μM. The binding mechanism of for Sn was confirmed by Fourier transform infrared analysis, NMR titrations, and mass (electrospray ionization) spectral analysis. Likewise, the proposed sensing mechanism was supported by quantum chemical calculations. Moreover, bioimaging studies demonstrated that the chemosensing probe is an effective fluorescent marker for the detection of Sn in living cells and zebrafish. Significantly, was able to discriminate between Sn in human cancer cells and Sn in normal live cells.
一种易于使用的比色法和荧光探针 4-((3-氯-1,4-二氧代-1,4-二氢萘-2-基)氨基)苯磺酰胺 () 被成功开发用于在水溶液中选择性和灵敏地检测 Sn。传感机制涉及在添加 Sn 时将 -C═O 还原为 中的 -C-OH 基团，从而引发荧光开启模式。在 0-62.5 μM 的范围内，荧光强度与 Sn 浓度之间呈现出更好的线性关系，检测限 (LOD) 为 0.115 μM。通过傅里叶变换红外分析、NMR 滴定和质量（电喷雾电离）光谱分析证实了 与 Sn 的结合机制。同样，基于量子化学计算支持了所提出的传感机制。此外，生物成像研究表明，化学传感探针 是一种有效的荧光标记物，可用于检测活细胞和斑马鱼中的 Sn。值得注意的是， 能够区分人癌细胞中的 Sn 和正常活细胞中的 Sn。
