School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
School of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai, China; Experiment Center of Teaching & Learning, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Food Chem. 2020 Jul 15;318:126358. doi: 10.1016/j.foodchem.2020.126358. Epub 2020 Feb 5.
Overdoses of SO and its derivatives (SO/HSO) in food or organisms are harmful to health. To detect SO/HSO, a novel NIR fluorescent probe 1, based upon the intramolecular charge transfer (ICT) mechanism, was developed. This probe was easily synthesized, and gave noticeable colorimetric and linear fluorescence changes at 690 nm after reaction with sulfite from 3.13 to 200 µM. Moreover, probe 1 displayed high sensitivity (LOD = 0.46 µM), excellent selectivity (among 13 kinds of anions and 3 kinds of biothiols) and quick response (within 30 min) towards SO/HSO. The SO/HSO sensing mechanism was confirmed as the Michael addition reaction. Furthermore, the probe showed wide applications for measuring SO/HSO in real samples, including sugar, tap water, wine and traditional Chinese medicine. The probe could also be used to detect SO/HSO in mitochondria of HepG2 cells and zebrafish, which suggested potential application for monitoring SO derivatives in clinical diagnostics.
食品或生物体中 SO 和其衍生物（SO/HSO）的过量摄入对健康有害。为了检测 SO/HSO，我们基于分子内电荷转移（ICT）机制开发了一种新型的近红外荧光探针 1。该探针易于合成，并且在与亚硫酸盐反应后在 690nm 处显示出明显的比色和线性荧光变化，从 3.13 到 200µM。此外，探针 1 对 SO/HSO 具有高灵敏度（LOD=0.46µM）、优异的选择性（在 13 种阴离子和 3 种生物硫醇中）和快速响应（在 30 分钟内）。SO/HSO 的传感机制被确认为迈克尔加成反应。此外，该探针在包括糖、自来水、葡萄酒和中药在内的实际样品中对 SO/HSO 的测定具有广泛的应用。该探针还可用于检测 HepG2 细胞和斑马鱼线粒体中的 SO/HSO，这表明其在临床诊断中监测 SO 衍生物具有潜在的应用价值。
