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一种基于BODIPY的铁荧光猝灭探针的合成与生物成像

Synthesis and bioimaging of a BODIPY-based fluorescence quenching probe for Fe.

作者信息

Leng Junqiang, Lan Xinyu, Liu Shuang, Jia Wenxuan, Cheng Wenshuai, Cheng Jianbo, Liu Zhenbo

机构信息

School of Chemistry and Chemical Engineering, Yantai University Yantai 264005 P. R. China

出版信息

RSC Adv. 2022 Aug 2;12(33):21332-21339. doi: 10.1039/d2ra00818a. eCollection 2022 Jul 21.

DOI:10.1039/d2ra00818a
PMID:35975086
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9344281/
Abstract

Iron is the main substance for maintaining life. Real-time determination of ferric ion (Fe) in living cells is of great significance for understanding the relationship of Fe concentration changes with various physiological and pathological processes. Fluorescent probes are suitable for the detection of trace metal ions in cells due to their low toxicity and high sensitivity. In this work, a boron-dipyrromethene-based fluorescent probe (BODIPY-CL) for selective detection of Fe was synthesized. The fluorescence emission of BODIPY-CL was determined at 516 nm. In a pH range of 1 to 10, the probe BODIPY-CL exhibits a quenching response to Fe. Meanwhile, BODIPY-CL showed a highly selective response to Fe compared with 16 kinds of metal ions. The stoichiometry ratio of BODIPY-CL bound to Fe was nearly 2 : 1. The fluorescence quenching response obtained by the sensor was linear with the Fe concentration in the range of 0-400 μM, and the detection limit was 2.9 μM. BODIPY-CL was successfully applied to image Fe in cells. This study provides a promising fluorescent imaging probe for further research on the physiological and pathological effects of Fe.

摘要

铁是维持生命的主要物质。实时测定活细胞中的铁离子（Fe）对于理解铁浓度变化与各种生理和病理过程之间的关系具有重要意义。荧光探针因其低毒性和高灵敏度而适用于细胞中痕量金属离子的检测。在本工作中，合成了一种用于选择性检测铁的基于硼二吡咯亚甲基的荧光探针（BODIPY-CL）。BODIPY-CL的荧光发射在516nm处测定。在pH值为1至10的范围内，探针BODIPY-CL对铁表现出猝灭响应。同时，与16种金属离子相比，BODIPY-CL对铁表现出高度选择性响应。BODIPY-CL与铁结合的化学计量比接近2∶1。该传感器获得的荧光猝灭响应与铁浓度在0-400μM范围内呈线性关系，检测限为2.9μM。BODIPY-CL成功应用于细胞中铁的成像。本研究为进一步研究铁的生理和病理效应提供了一种有前景的荧光成像探针。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/8b5bb1130091/d2ra00818a-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/12007d7d7168/d2ra00818a-s1.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/4a57dde8d0f1/d2ra00818a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/43a847893b36/d2ra00818a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/8cffb36fc6b2/d2ra00818a-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/8b5bb1130091/d2ra00818a-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/12007d7d7168/d2ra00818a-s1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/a3b6b1603141/d2ra00818a-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/7ac8b0df9e07/d2ra00818a-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/4a57dde8d0f1/d2ra00818a-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/43a847893b36/d2ra00818a-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/8cffb36fc6b2/d2ra00818a-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf8f/9344281/8b5bb1130091/d2ra00818a-f6.jpg

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