State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Centre for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai 200240, P. R. China.
Org Lett. 2021 Jan 1;23(1):37-41. doi: 10.1021/acs.orglett.0c03600. Epub 2020 Dec 7.
Alb29, an α-oxoamine synthase involved in albogrisin biosynthesis in MGR072, was characterized and responsible for the incorporation of l-glutamate to acyl-coenzyme A substrates. Combined with Alb29 and Mgr36 (an acyl-coenzyme A ligase), a one-pot enzymatic system was established to synthesize seven α-amino ketones. When these α-amino ketones were fed into the knockout strain Δ, respectively, the albogrisin analogs with different side chains were observed.
Alb29 是一种 α-氧胺合酶，参与 MGR072 中 albogrisin 的生物合成，负责将 l-谷氨酸掺入酰基辅酶 A 底物中。结合 Alb29 和 Mgr36（酰基辅酶 A 连接酶），建立了一锅酶促体系来合成七种 α-氨基酮。当将这些 α-氨基酮分别添加到 敲除菌株 Δ 中时，观察到具有不同侧链的 albogrisin 类似物。
